Combination of RT-LAMP and fluorescence spectroscopy using chemometric techniques for an ultra-sensitive and rapid alternative for the detection of SARS-CoV-2

Autor: Leticia Tessaro, Yhan da Silva Mutz, Carini Aparecida Lelis, Jelmir Craveiro de Andrade, Adriano Aquino, Pedro Panzenhagen, Alan Clavelland Ochioni, Italo Rennan Sousa Vieira, Carlos Adam Conte-Junior
Rok vydání: 2022
Předmět:
Zdroj: Analytical methods : advancing methods and applications. 14(47)
ISSN: 1759-9679
Popis: The increased spread of COVID-19 caused by SARS-CoV-2 has made it necessary to develop more efficient, fast, accurate, specific, sensitive and easy-to-use detection platforms to overcome the disadvantages of gold standard methods (RT-qPCR). Here an approach was developed for the detection of the SARS-CoV-2 virus using the loop-mediated isothermal amplification (LAMP) technique for SARS-CoV-2 RNA target amplification in samples of nasopharyngeal swabs. The discrimination between positive and negative SARS-CoV-2 samples was achieved by using fluorescence spectra generated by the excitation of the LAMP's DNA intercalator dye at
Databáze: OpenAIRE