Autor: |
Leticia Tessaro, Yhan da Silva Mutz, Carini Aparecida Lelis, Jelmir Craveiro de Andrade, Adriano Aquino, Pedro Panzenhagen, Alan Clavelland Ochioni, Italo Rennan Sousa Vieira, Carlos Adam Conte-Junior |
Rok vydání: |
2022 |
Předmět: |
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Zdroj: |
Analytical methods : advancing methods and applications. 14(47) |
ISSN: |
1759-9679 |
Popis: |
The increased spread of COVID-19 caused by SARS-CoV-2 has made it necessary to develop more efficient, fast, accurate, specific, sensitive and easy-to-use detection platforms to overcome the disadvantages of gold standard methods (RT-qPCR). Here an approach was developed for the detection of the SARS-CoV-2 virus using the loop-mediated isothermal amplification (LAMP) technique for SARS-CoV-2 RNA target amplification in samples of nasopharyngeal swabs. The discrimination between positive and negative SARS-CoV-2 samples was achieved by using fluorescence spectra generated by the excitation of the LAMP's DNA intercalator dye at |
Databáze: |
OpenAIRE |
Externí odkaz: |
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