Cytotoxicity testing of materials with limitedin vivo exposure is affected by the duration of cell-material contact
| Autor: | Gabriela Ciapetti, E. Verri, Lucia Savarino, Franca Savioli, Susanna Stea, A. Gori, Donatella Granchi, Lucio Montanaro |
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| Rok vydání: | 1998 |
| Předmět: |
Neutral red
Materials science Biocompatibility Cell Survival Silicones Biomedical Engineering Biocompatible Materials Pharmacology Biomaterials Mice chemistry.chemical_compound L Cells In vivo Materials Testing Animals Cytotoxic T cell Propidium iodide Coloring Agents Cytotoxicity Dental Impression Materials Amido Black Staining Dental impression chemistry Neutral Red Propidium Biomedical engineering |
| Zdroj: | Journal of Biomedical Materials Research. 42:485-490 |
| ISSN: | 1097-4636 0021-9304 |
| DOI: | 10.1002/(sici)1097-4636(19981215)42:4<485::aid-jbm2>3.0.co;2-7 |
| Popis: | Silicones for dental impression largely are used to record the geometry of hard and soft dental tissues. They are considered to be medical devices, and the assessment of cytotoxicity is a necessary step in the evaluation of their biocompatibility. Extracts of six addition-type and six condensation-type silicones have been tested with L929 cells according to the ISO 10993-Part 5 standard. The cytotoxicity was evaluated by three different methods: neutral red uptake, propidium iodide (PI) staining, and amido black staining. According to the selected specific assay, contact between cells and material extracts was maintained for 24 h in the first series of experiments; then, considering that in vivo application of these materials is restricted to a few minutes, additional experiments were performed after 1 h of cell/extract contact. Analysis of the results showed that the addition-type silicones are nontoxic even when tested after prolonged exposure of the cells to the materials while the condensation-type silicones were cytotoxic at 24 h of incubation. Nevertheless, harm to the patient actually could be negligible, considering its very short time of exposure in vivo. This is supported by our finding that most are not toxic after 1 h. We suggest that the experimental conditions of cytotoxicity testing have to be relevant to the in vivo situation; accordingly, the time of exposure should be designed carefully. |
| Databáze: | OpenAIRE |
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