No evidence for inositol 1,4,5-trisphosphate-dependent Ca2+ release in isolated fibers of adult mouse skeletal muscle
| Autor: | Laura Zúñiga Rodríguez, Victor-Hugo Hernandez Gonzalez, Bert Blaauw, Paola Del Piccolo, Francesca Solagna, Lisa Agatea, Tullio Pozzan, Stefano Schiaffino, Fabio Mammano |
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| Rok vydání: | 2012 |
| Předmět: |
EXPRESSION
medicine.medical_specialty Physiology Muscle Fibers Skeletal chemistry.chemical_element Mice Inbred Strains Uridine Triphosphate Inositol 1 4 5-Trisphosphate Calcium Biology MYOCYTES INSP(3) RECEPTORS PATHWAY 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine CHANNEL SIGNALS Internal medicine medicine Myocyte Animals Inositol 1 4 5-Trisphosphate Receptors Inositol Calcium Signaling Cells Cultured 030304 developmental biology Calcium signaling Calcium metabolism 0303 health sciences Photolysis Ryanodine receptor Communication Skeletal muscle TRANSIENTS Inositol trisphosphate receptor SARCOPLASMIC-RETICULUM LOCALIZE Endocrinology medicine.anatomical_structure chemistry CELLS 030217 neurology & neurosurgery SARCOPLASMIC-RETICULUM INSP(3) RECEPTORS CELLS EXPRESSION TRANSIENTS SIGNALS MYOCYTES LOCALIZE PATHWAY CHANNEL |
| Zdroj: | The Journal of General Physiology The Journal of General Physiology; Vol 140 |
| ISSN: | 1540-7748 |
| Popis: | The presence and role of functional inositol 1,4,5-trisphosphate (IP(3)) receptors (IP(3)Rs) in adult skeletal muscle are controversial. The current consensus is that, in adult striated muscle, the relative amount of IP(3)Rs is too low and the kinetics of Ca(2+) release from IP(3)R is too slow compared with ryanodine receptors to contribute to the Ca(2+) transient during excitation-contraction coupling. However, it has been suggested that IP(3)-dependent Ca(2+) release may be involved in signaling cascades leading to regulation of muscle gene expression. We have reinvestigated IP(3)-dependent Ca(2+) release in isolated flexor digitorum brevis (FDB) muscle fibers from adult mice. Although Ca(2+) transients were readily induced in cultured C2C12 muscle cells by (a) UTP stimulation, (b) direct injection of IP(3), or (c) photolysis of membrane-permeant caged IP(3), no statistically significant change in calcium signal was detected in adult FDB fibers. We conclude that the IP(3)-IP(3)R system does not appear to affect global calcium levels in adult mouse skeletal muscle. |
| Databáze: | OpenAIRE |
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