The Effect of Curcumin on Insulin Release in Rat-Isolated Pancreatic Islets
| Autor: | Hanan H. Ahmed, M.F. El-Asmar, Mohamed A Wassef, Essam G. El Nadi, Dina Sabry, Amira Hassouna, Ahmed T. Abdel Aziz, Eman Obaia, Laila A. Rashed, Mohamed T. Abdel Aziz |
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| Rok vydání: | 2010 |
| Předmět: |
endocrine system
medicine.medical_specialty Curcumin endocrine system diseases Metalloporphyrins medicine.medical_treatment Gene Expression Enzyme-Linked Immunosorbent Assay Islets of Langerhans chemistry.chemical_compound Internal medicine medicine Animals Insulin Pancreatic hormone DNA Primers Electrophoresis Agar Gel geography geography.geographical_feature_category Reverse Transcriptase Polymerase Chain Reaction business.industry Pancreatic islets Islet Rats Glucose medicine.anatomical_structure Endocrinology chemistry L-Glucose Hemin Cardiology and Cardiovascular Medicine Pancreas business Heme Oxygenase-1 |
| Zdroj: | Angiology. 61:557-566 |
| ISSN: | 1940-1574 0003-3197 |
| Popis: | Curcumin exerts a hypoglycemic action and induces heme-oxygenase-1 (HO-1). We evaluated the effect of curcumin on isolated islets of Langerhans and studied whether its action on insulin secretion is mediated by inducible HO-1. Islets were isolated from rats and divided into control islets, islets incubated in different curcumin concentrations, islets incubated in hemin, islets incubated in curcumin and HO inhibitor, stannous mesoporphyrin (SnMP), islets incubated in hemin and SnMP, islets incubated in SnMP only, and islets incubated in 16.7 mmol/L glucose. Heme-oxygenase activity, HO-1 expression, and insulin estimation was assessed. Insulin secretion, HO-1 gene expression and HO activity were significantly increased in islets incubated in curcumin, hemin, and glucose compared with controls. This increase in insulin secretion was significantly decreased by incubation of islets in SnMP. The action of curcumin on insulin secretion from the isolated islets may be, in part, mediated through increased HO-1 gene expression. |
| Databáze: | OpenAIRE |
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