Anti-tissue transglutaminase antibodies in inflammatory bowel disease: new evidence
| Autor: | Roberta Pica, Angelo Viscido, Marco Di Tola, M.C. Anania, L. Sabbatella, Antonio Picarelli, Paolo Paoluzi, Renzo Caprilli |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Adult
Serum Immunoglobulin A medicine.medical_specialty Tissue transglutaminase Clinical Biochemistry Severity of Illness Index Inflammatory bowel disease Gastroenterology Autoimmune Diseases Sprue Diagnosis Differential Feces Crohn Disease Antigen anti-endomysial celiac sprue crohn's disease crohn’s disease transglutaminase ulcerative colitis Internal medicine medicine Humans Crohn's disease Transglutaminases biology business.industry digestive oral and skin physiology Biochemistry (medical) nutritional and metabolic diseases General Medicine Inflammatory Bowel Diseases medicine.disease Ulcerative colitis digestive system diseases Antibodies Anti-Idiotypic Celiac Disease Case-Control Studies Immunology biology.protein Antibody business |
| Zdroj: | Clinical Chemistry and Laboratory Medicine (CCLM). 42 |
| ISSN: | 1437-4331 1434-6621 |
| DOI: | 10.1515/cclm.2004.225 |
| Popis: | Anti-tissue transglutaminase, previously held to be identical to anti-endomysial antibodies in celiac sprue, has been reported in inflammatory bowel disease patients. To investigate these data further, we evaluated serum and intestinal anti-tissue transglutaminase in inflammatory bowel disease patients, with respect to the Crohn’s disease activity index and the integrated disease activity index. Study population comprised: 49 patients with Crohn’s disease and 29 patients with ulcerative colitis; 45 patients with celiac sprue and 85 autoimmune patients as disease controls; and 58 volunteers as healthy controls. Immunoglobulin A (IgA) anti-recombinant human tissue transglutaminase and anti-endomysial antibody detection in sera and fecal supernatants were performed. Adsorption of positive sera with recombinant human tissue transglutaminase were also performed. Marked increased anti-tissue transglutaminase concentrations were found in celiac sprue, while low-positive values were also found in Crohn’s disease and ulcerative colitis. Anti-endomysial antibodies were detectable only in celiac sprue. Antigen adsorption resulted in a significant reduction of the anti-tissue transglutaminase either in celiac sprue or inflammatory bowel disease sera. A significant correlation between anti-tissue transglutaminase and Crohn’s disease activity index or integrated disease activity index scores was found. Anti-tissue transglutaminase was also detectable in fecal supernatants from inflammatory bowel disease patients. Data highlight that both circulating and intestinal anti-tissue transglutaminases are detectable in inflammatory bowel disease, and that they are related to disease activity. These features underline that, in addition to anti-tissue transglutaminase, an anti-endomysial antibody test is necessary in the diagnostic work-up of celiac sprue, especially in patients with known inflammatory bowel disease. |
| Databáze: | OpenAIRE |
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