Production and characterization of agonistic monoclonal antibodies against chicken CD40
| Autor: | Jocelyn Bray, Waithaka Mwangi, Nancy H. Ing, Suryakant D. Waghela, Chang-Hsin Chen, Daad Abi-Ghanem, Jackson L. McReynolds, Leo M. Njongmeta, Luc Berghman |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.drug_class
CD40 Ligand Immunology Ligands Nitric Oxide Monoclonal antibody Avian Proteins Affinity maturation Immune system Adjuvants Immunologic Antigen medicine Animals CD40 Antigens CD154 Cells Cultured Cell Proliferation B-Lymphocytes CD40 biology Macrophages Antibodies Monoclonal hemic and immune systems Molecular biology Protein Structure Tertiary Immunoglobulin class switching biology.protein Antibody Genetic Engineering Chickens Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 34:1139-1143 |
| ISSN: | 0145-305X |
| DOI: | 10.1016/j.dci.2010.06.014 |
| Popis: | CD40 is mainly expressed by professional antigen-presenting cells (APCs). Its ligand, CD40L, is transiently expressed on activated CD4(+) T-cells. CD40-CD40L interactions mediate T-cell help to APCs and provide crucial signals for affinity maturation and B-cell class switching. In mammals, agonistic monoclonal anti-CD40 antibodies (mAbs) mimic the effects of CD40L on APCs, leading to enhanced T-cell priming and expansion, increased antibody production and class switching. In this study, we describe agonistic anti-chicken CD40 mAb 2C5. This mAb detected CD40 on primary chicken B-cells and macrophages, DT40 B-cells, and HD11 macrophages, induced NO synthesis in HD11 macrophages, and stimulated DT40 B-cell proliferation. These observations demonstrated at least partial functional equivalence of 2C5 to chicken CD154. This mAb may therefore constitute a new tool to study the role of CD40 in the chicken immune system, and its agonistic effects suggest that it could also be used as an adjuvant. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect