Effective adjuvants for the induction of antigen-specific delayed-type hypersensitivity
| Autor: | Jon R. Ward, Jory R. Baldridge |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cellular immunity medicine.drug_class Injections Subcutaneous T cell medicine.medical_treatment Monophosphoryl Lipid A chemical and pharmacologic phenomena Biology Immunostimulant Microbiology Epitopes Mice Adjuvants Immunologic Antigen medicine Animals Hypersensitivity Delayed Antigens Bacterial Immunity Cellular Mice Inbred BALB C Mice Inbred ICR General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health Listeria monocytogenes Mice Inbred C57BL Infectious Diseases medicine.anatomical_structure Vaccines Inactivated Delayed hypersensitivity Bacterial Vaccines Immunology Molecular Medicine Female Adjuvant Injections Intraperitoneal CD8 |
| Zdroj: | Vaccine. 15:395-401 |
| ISSN: | 0264-410X |
| Popis: | Vaccines utilizing poorly immunogenic subunit antigens are dependent upon adjuvants to drive the appropriate T cell responses. In an effort to determine the ability of several adjuvants to promote cell-mediated immunity (CMI), we assessed delayed-type hypersensitivity (DTH) in mice inoculated with heat-killed Listeria monocytogenes (HKLM) vaccines. The vaccines were formulated as oil-in-water emulsions containing one or more of the following bacterial-derived immunostimulators: MPL immunostimulant, a monophosphoryl lipid A preparation, synthetic trehalose dicorynomycolate (TDCM) and Mycobacterium phlei cell wall skeleton (CWS). Oil-in-water emulsions containing HKLM without adjuvants did not induce DTH responsiveness in mice. The incorporation of TDCM, or MPL plus TDCM and/or CWS to the formulation enabled the HKLM vaccine to stimulate CMI characterized by DTH responsiveness. Following antigen challenge the resulting increases in footpad thickness ranged from 15-20% and were comparable to the DTH driven by complete Freund's adjuvant. Adjuvants composed of MPL/TDCM and MPL/TDCM/CWS induced responses equivalent to those measured in mice immunized with viable L. monocytogenes, and the responses remained at these levels for at least 2 months. Furthermore, in vivo depletion of CD4+ T cells, but not CD8+ T cells, abrogated the induction and expression of DTH, indicating that the response is mediated by CD4+ T cells. |
| Databáze: | OpenAIRE |
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