Multipotent cell lineages in early mouse development depend on SOX2 function
| Autor: | Lidia Pérez, Robin Lovell-Badge, Silvia K. Nicolis, Larysa H. Pevny, Ariel A. Avilion, Nigel Vivian |
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| Přispěvatelé: | Avilion, A, Nicolis, S, Pevny, L, Perez, L, Vivian, N, Lovell Badge, R |
| Rok vydání: | 2003 |
| Předmět: |
Male
Fibroblast Growth Factor Transcription Factor Ectoderm Mice Pregnancy Maternal-Fetal Exchange reproductive and urinary physiology Multipotent Stem Cell Nuclear Protein Genetics Microscopy Confocal Trophoblast Gene Expression Regulation Developmental Nuclear Proteins Chorion Trophoblasts DNA-Binding Proteins Phenotype medicine.anatomical_structure Gene Targeting embryonic structures Female Endoderm Research Paper Heterozygote animal structures DNA-Binding Protein Rex1 SOXB1 Transcription Factor Biology Cell fate determination Embryonic and Fetal Development HMGB Proteins medicine Animals Cell Lineage Crosses Genetic Animal Chimera Multipotent Stem Cells SOXB1 Transcription Factors Nanog Homeobox Protein Embryo Transfer Embryonic stem cell Fibroblast Growth Factors Blastocyst Epiblast Genes Lethal Octamer Transcription Factor-3 Transcription Factors Developmental Biology |
| Zdroj: | Genes & Development. 17:126-140 |
| ISSN: | 1549-5477 0890-9369 |
| DOI: | 10.1101/gad.224503 |
| Popis: | Each cell lineage specified in the preimplantation mammalian embryo depends on intrinsic factors for its development, but there is also mutual interdependence between them. OCT4 is required for the ICM/epiblast lineage, and at transient high levels for extraembryonic endoderm, but also indirectly through its role in regulatingFgf4expression, for the establishment and proliferation of extraembryonic ectoderm from polar trophectoderm. The transcription factor SOX2 has also been implicated in the regulation ofFgf4expression. We have used gene targeting to inactivateSox2, examining the phenotypic consequences in mutant embryos and in chimeras in which the epiblast is rescued with wild-type ES cells. We find a cell-autonomous requirement for the gene in both epiblast and extraembryonic ectoderm, the multipotent precursors of all embryonic and trophoblast cell types, respectively. However, an earlier role within the ICM may be masked by the persistence of maternal protein, whereas the lack of SOX2 only becomes critical in the chorion after 7.5 days postcoitum. Our data suggest that maternal components could be involved in establishing early cell fate decisions and that a combinatorial code, requiring SOX2 and OCT4, specifies the first three lineages present at implantation. |
| Databáze: | OpenAIRE |
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