Ponticoccus marisrubri sp. nov., a moderately halophilic marine bacterium of the family Rhodobacteraceae
| Autor: | Di Liu, Xiaoyan Dong, Qin Xiong, Weibing Xun, Ruifu Zhang, Guishan Zhang, Xiuzhu Dong, Mohamed F. Haroon, Ulrich Stingl |
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| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial 0301 basic medicine food.ingredient Ubiquinone Saudi Arabia medicine.disease_cause Microbiology 03 medical and health sciences food Marine bacteriophage RNA Ribosomal 16S medicine Seawater Rhodobacteraceae Ponticoccus Indian Ocean Phospholipids Phylogeny Ecology Evolution Behavior and Systematics Base Composition biology Phylogenetic tree Strain (chemistry) Fatty Acids Nucleic Acid Hybridization Phosphatidylglycerols Sequence Analysis DNA General Medicine biology.organism_classification 16S ribosomal RNA Halophile Bacterial Typing Techniques 030104 developmental biology Ponticoccus litoralis |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 67:4358-4364 |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | Strain SJ5A-1T, a Gram-stain-negative, coccus-shaped, non-motile, aerobic bacterium, was isolated from the brine–seawater interface of the Erba Deep in the Red Sea, Saudi Arabia. The colonies of strain SJ5A-1T have a beige to pale-brown pigmentation, are approximately 0.5–0.7 µm in diameter, and are catalase and oxidase positive. Growth occurred optimally at 30–33 °C, pH 7.0–7.5, and in the presence of 9.0–12.0 % NaCl (w/v). Phylogenetic analysis of the 16S rRNA gene indicates that strain SJ5A-1T is a member of the genus Ponticoccus within the family Rhodobacteraceae . Ponticoccus litoralis DSM 18986T is the most closely related described species based on 16S rRNA gene sequence identity (96.7 %). The DNA–DNA hybridization value between strain SJ5A-1T and P. litoralis DSM 18986T was 36.7 %. The major respiratory quinone of strain SJ5A-1T is Q-10; it predominantly uses the fatty acids C18 : 1 (54.2 %), C18 : 0 (11.2 %), C16 : 0 (8.6 %), 11-methyl C18 : 1 ω7c (7.7 %), C19 : 0cyclo ω8c (3.3 %), and C12 : 1 3-OH (3.5 %), and its major polar lipids are phosphatidylethanolamine, phosphatidylglycerol, phosphocholine, an unknown aminolipid, an unknown phospholipid and two unknown lipids. The genome draft of strain SJ5A-1T as presented here is 4 562 830 bp in size and the DNA G+C content is 68.0 mol%. Based on phenotypic, phylogenetic and genotypic data, strain SJ5A-1T represents a novel species in the genus Ponticoccus , for which we propose the name Ponticoccus marisrubri sp. nov. The type strain of P. marisrubri is SJ5A-1T (=JCM 19520T=ACCC19863T). |
| Databáze: | OpenAIRE |
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