Grape (Vitis vinifera) VvDOF3 functions as a transcription activator and enhances powdery mildew resistance
| Autor: | Ke-Ke Yu, Yu-Tong Wan, Da-Long Guo, Ze-Ling Jiao, Lu Bian, Yi-He Yu, Guo-Hai Zhang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Plant Science Cyclopentanes Plant disease resistance Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Arabidopsis Genetics Arabidopsis thaliana Vitis Oxylipins Transcription factor Disease Resistance Plant Diseases Plant Proteins Zinc finger Jasmonic acid fungi food and beverages biology.organism_classification Cell biology 030104 developmental biology chemistry Salicylic Acid Salicylic acid Powdery mildew 010606 plant biology & botany Transcription Factors |
| Zdroj: | Plant physiology and biochemistry : PPB. 143 |
| ISSN: | 1873-2690 |
| Popis: | DOF proteins are plant-specific transcription factors that play vital roles in plant development and defense responses. However, DOFs have primarily been investigated in model plants, and fairly limited research has been performed on grape (Vitis vinifera). In this study, we isolated and characterized a C2-C2 zinc finger structural DOF gene, VvDOF3, from the grape cultivar Jingxiu. The VvDOF3 protein showed nuclear localization and transcriptional activation ability, indicating that it functions as a transcription factor. The VvDOF3 gene was rapidly induced by exogenous salicylic acid (SA), jasmonic acid (JA), and powdery mildew infection. Overexpression of VvDOF3 in Arabidopsis thaliana enhanced resistance to Golovinomyces cichoracearum. Expression of the SA-responsive defense-related gene PR1 and the concentration of SA were up-regulated in transgenic Arabidopsis plants overexpressing VvDOF3. Together, these data suggest that VvDOF3 functions as a transcription factor in grape and enhances powdery mildew resistance through the SA signaling pathway. |
| Databáze: | OpenAIRE |
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