Expression of Interactive Genes Associated with Apoptosis and Their Prognostic Value for Ovarian Serous Adenocarcinoma

Autor: Ki Hyung Kim, Man Soo Yoon, Ari Kim, Wankyu Eo, Dong Soo Suh, Ji Young Lee, Kyusik Shin
Rok vydání: 2016
Předmět:
0301 basic medicine
Oncology
medicine.medical_specialty
endocrine system diseases
Medicine (miscellaneous)
Apoptosis
Ovarian Serous Adenocarcinoma
DNA-Activated Protein Kinase
Kaplan-Meier Estimate
General Biochemistry
Genetics and Molecular Biology
AKT3
law.invention
03 medical and health sciences
0302 clinical medicine
law
Internal medicine
Internal Medicine
medicine
Humans
Pharmacology (medical)
Genetics (clinical)
Neoplasm Staging
Ovarian Neoplasms
Glycogen Synthase Kinase 3 beta
Reverse Transcriptase Polymerase Chain Reaction
business.industry
Nuclear Proteins
Cancer
Middle Aged
Cell cycle
Prognosis
medicine.disease
Immunohistochemistry
Cystadenocarcinoma
Serous
Gene Expression Regulation
Neoplastic
030104 developmental biology
CA-125 Antigen
030220 oncology & carcinogenesis
Reviews and References (medical)
Suppressor
Female
Neoplasm Grading
Tumor Suppressor Protein p53
business
Ovarian cancer
Proto-Oncogene Proteins c-akt
Zdroj: Advances in Clinical and Experimental Medicine. 25:513-521
ISSN: 1899-5276
DOI: 10.17219/acem/62540
Popis: Background Malignant ovarian tumor is one of the leading causes of worldwide cancer death. It is usually characterized by insidious onset and late diagnosis because of the absence of symptoms, allowing ovarian cancer cases to progress rapidly and become unresectable. The tumor suppressor, p53, plays an important role in regulating cell cycles and apoptosis. p53 is regulated by several molecules, and it interacts with other apoptotic proteins. Objectives To compare the prognosis of ovarian serous carcinoma and evaluate the expression of DNA-PKcs, Akt3, GSK-3β, and p53 in cancerous cells. Material and methods DNA-PKcs, Akt3, GSK-3β, and p53 expression levels were scored using immunohistochemistry staining of tissue samples from 132 women with ovarian serous adenocarcinoma. Expression was confirmed by real-time RT-PCR. Analyses were stratified by age, tumor grades, cancer stages and serum CA 125 levels. Results Significant differences in DNA-PKcs, Akt3, and p53 expression were observed between participants with different stages and tumor grades of ovarian serous adenocarcinoma. DNA-PKcs and p53 expression increased along with increasing tumor grade. Meanwhile, DNA-PKcs, Akt3, and p53 expression increased along with increasing cancer stage, and with a decrease in 5-year overall survival rate. Conclusions This study shows that elevated expression of DNA-PKcs, Akt3, and p53 in ovarian serous adenocarcinoma tissues are an indication of more advanced disease and worse prognosis.
Databáze: OpenAIRE
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