Characterization of antioxidant properties of natural killer-enhancing factor-B and induction of its expression by hydrogen peroxide
| Autor: | Anthony T. Kim, Theodore A. Sarafian, Hungyi Shau |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Cytotoxicity
Immunologic Antioxidant medicine.medical_treatment Oxidative phosphorylation Toxicology medicine.disease_cause Transfection Complement factor B Antioxidants Umbilical Cord chemistry.chemical_compound tert-Butylhydroperoxide medicine Humans Buthionine sulfoximine RNA Messenger Enzyme Inhibitors Hydrogen peroxide Buthionine Sulfoximine Cells Cultured Heat-Shock Proteins Pharmacology Sulfhydryl Reagents Blood Proteins Hydrogen Peroxide Peroxiredoxins Methylmercury Compounds Blotting Northern Oxidants Peroxides Endothelial stem cell Oxidative Stress chemistry Biochemistry Gene Expression Regulation Peroxidases Endothelium Vascular Reactive Oxygen Species Oxidative stress |
| Zdroj: | Toxicology and applied pharmacology. 147(1) |
| ISSN: | 0041-008X |
| Popis: | Natural killer-enhancing factor B (NKEF-B) belongs to a highly conserved family of recently discovered antioxidants. The role of NKEF-B as an antioxidant was demonstrated by its protection of transfected cells to oxidative damage by hydrogen peroxide. To further characterize the antioxidant properties of NKEF-B, we compared the sensitivity of a human endothelial cell line ECV304 and its transfectant, B/1 that hyperexpresses NKEF-B, to various oxidants. In addition, we investigated the changes in the expression of NKEF-B mRNA upon oxidative stress. We found that B/1 was significantly more resistant than the control cells to the oxidative stresses caused by t-butyl hydroperoxide (t-BHP) and methyl mercury (MeHg). In contrast, there was no difference in the sensitivity of B/1 and the control cells to sulfhydryl reactive agents, diethyl maleate and diamide. B/1 was also as sensitive as the control cells to buthionine sulfoximine. The expression of NKEF-B mRNA was induced when the parental cell line ECV304 was treated with 2 mm HP. The induction reached a maximum level around 2 hr and decreased to the basal level around 4 hr. NKEF-A mRNA was not induced by HP. These results demonstrate antioxidant activities of NKEF-B toward prooxidants such as alkyl hydroperoxide and MeHg. Together with its antioxidant activity, the induction of NKEF-B by HP indicates that NKEF-B is an important oxidative stress protein providing protection against a variety of xenobiotic toxic agents. |
| Databáze: | OpenAIRE |
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