RELEASE OF TRAPPED MARKER FROM LIPOSOMES BY THE ACTION OF PURIFIED COMPLEMENT COMPONENTS

Autor:	S. C. Kinsky, J. A. Haxby, Hans J. Müller-Eberhard, O. Götze
Rok vydání:	1969
Předmět:	Liposome Erythrocytes Sheep Multidisciplinary biology Cell Membrane Biological membrane Complement System Proteins Lipids Complement components Cell membrane Glucose Membrane medicine.anatomical_structure Biochemistry biology.protein medicine Animals Biological Sciences: Biochemistry Antibody
Zdroj:	Proceedings of the National Academy of Sciences. 64:290-295
ISSN:	1091-6490 0027-8424
DOI:	10.1073/pnas.64.1.290
Popis:	Liposomes containing trapped glucose marker were prepared from the chloroform-soluble fraction of sheep erythrocyte membranes. These liposomes release glucose when incubated with rabbit anti-sheep erythrocyte serum and a source of complement. Experiments with purified human complement components show that loss of marker is absolutely dependent on the presence of components 2 and 8. An absolute requirement for component 9 cannot be demonstrated, although it stimulates glucose release from the liposomes. These results establish a parallelism between the response of biological membranes and liposomal membranes to antibody and complement.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7a9cacde75558556fc4231533acd802 https://doi.org/10.1073/pnas.64.1.290 Zobrazit plný text záznamu