New attempt of preoperative differential diagnosis of thyroid neoplasms by telomerase activity measurement
| Autor: | Hirofumi Ami, Hitoshi Onogi, Izumi Nakamura, Shinichi Suzuki, Toshihiko Fukushima, Seiichi Takenoshita |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adenoma
Cancer Research Pathology medicine.medical_specialty endocrine system diseases Biology medicine.disease_cause Malignancy Benign tumor Thyroid carcinoma Diagnosis Differential Adenocarcinoma Follicular medicine Carcinoma Humans Thyroid Neoplasms Telomerase Thyroid neoplasm Goiter Thyroid Cancer General Medicine Telomere medicine.disease Carcinoma Papillary medicine.anatomical_structure Oncology Carcinoma Medullary Adenocarcinoma |
| Zdroj: | Oncology reports. 9(3) |
| ISSN: | 1021-335X |
| Popis: | Telomerase activity (TA) has been shown highly expressed in various solid malignant neoplasms, but only rarely in benign tumor or normal tissues. Most reports examined TA by the qualitative method, telomeric repeat amplification protocol (TRAP) assay. We examined TA in tissues of thyroid neoplasm by quantitative method to establish whether TA was a useful marker for preoperative differential diagnosis of thyroid tumors. TA was measured by the quantitative method, TRAP assay, fluorescence based TRAP method in 32 frozen tissues of thyroid tumors (17 malignant, 15 benign). Cut-off values in TA were 0 unit and 9 units from mean +/- 2SD of TA in benign thyroid tumors. Mean (SD) TA levels in benign thyroid tumors and thyroid carcinomas were 2.06 (3.58) units and 30.5 (38.2), respectively. Mean TA of carcinomas was significantly higher than that of benign disease in thyroid tumors (p=0.0092). The sensitivity of the malignancy was 64.7% (cut-off |
| Databáze: | OpenAIRE |
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