SNPs on Chips: The Hidden Genetic Code in Expression Arrays
| Autor: | William E. Bunney, Terence P. Speed, Stanley J. Watson, Edward G. Jones, Huda Akil, Margit Burmeister, Fan Meng, Elzbieta Sliwerska |
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| Rok vydání: | 2007 |
| Předmět: |
Genetics
Analysis of Variance Polymorphism Genetic Genotype Microarray Gene Expression Single-nucleotide polymorphism Biology Catechol O-Methyltransferase Genetic code Genetic determinism Genetic Code Gene expression Humans Gene Biological Psychiatry Oligonucleotide Array Sequence Analysis Genetic association |
| Zdroj: | Biological Psychiatry. 61:13-16 |
| ISSN: | 0006-3223 |
| DOI: | 10.1016/j.biopsych.2006.01.023 |
| Popis: | Gene expression microarray analysis in postmortem brains is one of the fastest growing fields of psychiatric research. Here we show that common polymorphisms (SNPs) present on probe sets can masquerade as significant "gene expression" differences. After first observing this artifact in the Catechol-O-methyl transferase (COMT) gene, we replicate the finding in two additional genes predicted to show this artifact. Many Affymetrix chips contain thousands of SNPs that are both common and in the central probe region affecting hybridization, and thus have the potential to confound expression analysis. |
| Databáze: | OpenAIRE |
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