Effects of 4-Hydroxy-2-Nonenal, a Major Lipid Peroxidation-Derived Aldehyde, and N-Acetylcysteine on the Cyclooxygenase-2 Expression in Human Uterine Myometrium
| Autor: | Takeshi Kanagawa, Kumiko Temma-Asano, Takuji Tomimatsu, Koichiro Shimoya, Ekaterine Tskitishvili, Tadashi Kimura, Tateki Tsutsui, Yuichiro Nakai, Yang Sil Chang, Takafumi Nakamura |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Gene Expression Prostaglandin medicine.disease_cause Chorioamnionitis 2-Nonenal Dinoprostone Tissue Culture Techniques Lipid peroxidation Acetylcysteine chemistry.chemical_compound Obstetric Labor Premature Pregnancy Internal medicine medicine Humans RNA Messenger reproductive and urinary physiology Aldehydes biology Myometrium Obstetrics and Gynecology medicine.disease Epoprostenol Endocrinology Reproductive Medicine Biochemistry chemistry Cyclooxygenase 2 biology.protein Female Cyclooxygenase Oxidative stress medicine.drug |
| Zdroj: | Gynecologic and Obstetric Investigation. 72:37-42 |
| ISSN: | 1423-002X 0378-7346 |
| Popis: | Background: Chorioamnionitis is one of the important causes of preterm labor. Preterm labor with chorioamnionitis is associated with oxidative stress. We reported that 4-hydroxy-2-nonenal (4-HNE), a major end product of oxidative fatty acid metabolism, is accumulated in the placenta with chorioamnionitis. The aim of this study was to confirm the effect of 4-HNE on cyclooxygenase-2 (COX-2) and prostaglandin (PG) induction in the uterine myometrial tissues. We also examined the effect of N-acetylcysteine (NAC) on 4-HNE-induced COX-2 expression. Methods: Uterine myometrial tissues were obtained from 5 patients. One of them underwent elective cesarean section without labor, and 4 of them underwent hysterectomy because of placental previa or atonic bleeding. We stimulated the uterine myometrial tissues with 4-HNE. In addition, the tissues were pretreated with NAC before 4-HNE treatment. The expression of COX-2 mRNA was observed by real-time PCR. PGE2 and prostacyclin release into the supernatants of the tissue cultures was measured by ELISA. Results: 4-HNE induced the COX-2 mRNA expression and PGE2 production in the uterine myometrial tissue culture in a dose-dependent and time-dependent manner. NAC inhibited 4-HNE-induced COX-2 expression. Conclusion: 4-HNE may play an important role in preterm labor. NAC might be protective against preterm labor under oxidative stress. |
| Databáze: | OpenAIRE |
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