Anti-inflammatory effects of prunin on UVB-irradiated human keratinocytes
| Autor: | Eun Ju Na, Ji Young Ryu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Antioxidant medicine.medical_treatment 030106 microbiology Flavonoid Prunin 03 medical and health sciences chemistry.chemical_compound Dichlorofluorescein Anti-inflammation lcsh:Dermatology medicine Viability assay Free-radical theory of aging chemistry.chemical_classification Chemistry General Medicine lcsh:RL1-803 Molecular biology HaCaT 030104 developmental biology HaCaT keratinocyte Tumor necrosis factor alpha Reactive oxygen species Ultraviolet B |
| Zdroj: | Biomedical Dermatology, Vol 2, Iss 1, Pp 1-6 (2018) |
| ISSN: | 2398-8460 |
| DOI: | 10.1186/s41702-018-0024-9 |
| Popis: | Background The flavonoid prunin is a flavanone glycoside found in cherry trees, including the flowering cherry Prunus yedoensis Matsumura (Rosacea). Although this compound has been studied for its antioxidant, anti-bacterial, and blood-sugar-lowering effects, no studies address its use in cosmetics. This study investigates whether prunin exhibits anti-inflammatory effects in cells exposed to ultraviolet B (UVB) radiation. Methods The effects of prunin were assessed by measuring cell viability using the water-soluble tetrazolium salt-1 assay and free radical damage using the dichlorofluorescein diacetate assay as well as by quantitative real-time PCR. Results UVB-induced decrease in cell viability diminished by pretreatment with prunin in a concentration-dependent manner. Intracellular reactive oxygen species (ROS) quantitative analysis revealed that the expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), which is associated with the inflammatory response, and mRNA expression of interleukin-6 (IL-6), interleukin-8 (IL-8), cyclooxygenase-2 (COX2), tumor necrosis factor-alpha (TNF-α), and protease-activated receptor (PAR2) decreased with prunin pretreatment in a concentration-dependent manner. Conclusions Prunin increased the survival rate of UVB-treated human keratinocyte HaCaT cells. Prunin protected the HaCaT cells, eliminated ROS, and demonstrated anti-inflammatory effects. Thus, prunin is worthy of investigation for use as a cosmetic ingredient that protects the skin and has anti-inflammatory effects. |
| Databáze: | OpenAIRE |
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