Tongxinluo reduces brain edema and inhibits post-ischemic inflammation after middle cerebral artery occlusion in rats
| Autor: | Min Cai, Jingsi Zhang, Lili Wang, Wen Zhang, Jun Xiang, Dingfang Cai, Zhennian Zhang, Xiao-Ling Song, Zhonghai Yu, Wen-Wei Li |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Ischemia Brain Edema Inflammation Pharmacology HMGB1 Brain Ischemia Rats Sprague-Dawley Brain ischemia 03 medical and health sciences 0302 clinical medicine Drug Discovery Animals Medicine HMGB1 Protein Microglia biology Tumor Necrosis Factor-alpha business.industry Therapeutic effect NF-kappa B Brain Infarction Middle Cerebral Artery medicine.disease Rats Toll-Like Receptor 4 030104 developmental biology medicine.anatomical_structure TLR4 biology.protein Tumor necrosis factor alpha medicine.symptom business 030217 neurology & neurosurgery Drugs Chinese Herbal |
| Zdroj: | Journal of Ethnopharmacology. 181:136-145 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2016.01.026 |
| Popis: | Ethnopharmacological relevance Tongxinluo (TXL), a widely used traditional Chinese medicine, has been proved multiple therapeutic effects in cerebral ischemic infraction. The purpose of this study was to investigate the protective effects of TXL on the brain edema and post-ischemic inflammatory response. Materials and Methods Middle cerebral artery occlusion in the rat was used as the ischemia model. Rats were treated with TXL. In the first stage, the best dosage was chosen based on functional assessment and infarct size. In the second stage, rats were randomly divided into 5 groups: sham control (sham), ischemia and reperfusion (IR) 24 h, TXL24h, I/R72h, TXL72h. TXL(1.6 g/kg/day) administration was pre-performed for 3 days in TXL groups, and was post-performed for 24 h (TXL24h group) or 72 h (TXL72h group). Brain edema was measured by water content, MRI and AQP4 expression. Iba1, HMGB1, TLR4, NF-κB expression were examined by immunofluorescence staining or Western blot. TNF-α was determined by enzyme-linked immunosorbent assay. Results High dose (1.6 g/kg/day) of TXL remarkably reduced neurological deficit scores and cerebral infarct area. Compared with those results of I/R24h group, pre-post treatment with TXL for 3 days decreased brain water content, down-regulated AQP4 expression, lowered relative signal intensity of T2WI, reduced lesion volume ratio, and inhibited the activation of microglia, HMGB1, TLR4, NF-κB and TNF-α. Conclusions These results indicated that the TXL pre-post treatment for 3 days could be an effective therapy for brain ischemia by inhibiting the development of brain edema and post-ischemic inflammation. |
| Databáze: | OpenAIRE |
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