Principal component analysis of the biological characteristics of entomopathogenic fungi in nutrient-limited and cuticle-based media
| Autor: | Alejandro Angel-Cuapio, César Andrés Ángel-Sahagún, Yazmin Colin-Bonifacio, Roberto Montesinos-Matías, Rosa Elia García-García, Armando Ordaz-Hernández, Hugo César Arredondo-Bernal |
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| Rok vydání: | 2020 |
| Předmět: |
food.ingredient
Insecta Beauveria bassiana Virulence Bassiana Applied Microbiology and Biotechnology Conidium 03 medical and health sciences food Agar Animals Pest Control Biological 030304 developmental biology 0303 health sciences Principal Component Analysis biology 030306 microbiology fungi General Medicine Nutrients Spores Fungal biology.organism_classification Yeast Culture Media Horticulture Germination Hypocreales Potato dextrose agar Insect Proteins |
| Zdroj: | Journal of basic microbiologyREFERENCES. 61(2) |
| ISSN: | 1521-4028 |
| Popis: | Media formulated with insect cuticle (0.5% and 1%; Sphequit Sph®), with a reduction in nutrients (¼ Sabouraud dextrose agar + yeast [SDAY]) and commercial media (potato dextrose agar, Sabouraud dextrose agar) were evaluated for the cultivation of Beauveria bassiana, Cordyceps javanica (Isaria javanica [Bally] Samson & Hywel-Jones), and Metarhizium robertsii. By using principal component analysis, it was determined that the ¼ SDAY and Sph formulations have greater advantages than commercial media for the development of fungi. The ¼ SDAY and Sph (0.5% and 1%) improved hydrophobicity, radial growth rate, germination, conidia yield, and virulence in B. bassiana; in M. robertsii, they favored conidia yield, germination, and virulence, and in C. javanica, the ¼ SDAY and Sph 0.5% media enhanced conidia yield, germination, radial growth rate, and virulence. We suggest that these formulations are an alternative to commercial culture media as they are cheaper and appropriate to improve the growth characteristics and virulence of the three strains evaluated. Some applications of culture media are suggested, and the importance of multivariate analysis as an exploratory tool to carry out the choice of culture media in a suitable way for the development of mycoinsecticides is also discussed. |
| Databáze: | OpenAIRE |
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