Effects of Bovine β-Casein (1-28) and Its Chemically Synthesized Partial Fragments on Proliferative Responses and Immunoglobulin Production in Mouse Spleen Cell Cultures
| Autor: | Hajime Otani, Yasuhito Tashiro, Takehito Watanabe |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Lipopolysaccharide
Immunoglobulins Lymphocyte Activation Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Mice chemistry.chemical_compound Adjuvants Immunologic Casein Amide Animals Amino Acid Sequence Lymphocytes Phosphorylation Molecular Biology Cells Cultured biology Immunochemistry Organic Chemistry Mouse Spleen Caseins General Medicine Molecular biology Peptide Fragments chemistry β casein Cell culture Concanavalin A biology.protein Cattle Mitogens Antibody Spleen Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 65:2489-2495 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.65.2489 |
| Popis: | Effects of bovine beta-casein (1-28) having a phosphoserine-rich region (Glu14-SerP-Leu-SerP-SerP-SerP-Glu-Glu21) and its chemically synthesized partial fragments on proliferation of lymphocytes and immunoglobulin production were investigated in mouse spleen cell cultures. The parent fragment 1-28 and all fragments containing SerP-Leu-SerP and/or SerP-SerP-SerP had a significant mitogenic effect, stimulated proliferation of lymphocytes induced by lipopolysaccharide, phytohemagglutinin, or concanavalin A, and increased immunoglobulin (IgG + IgM + IgA) or IgA levels in the cell cultures. In contrast, dephosphorylated beta-casein (14-21) and SerP-SerP amide had hardly any immunoregulatory activity. On the other hand, SerP-Leu-SerP amide reacted little with antibodies specific to bovine beta-casein (1-28), but beta-casein (14-21), and SerP-SerP-SerP amide obviously reacted with the antibody. These results confirm that the immunoregulatory activity of casein phosphopeptides is attributable to SerP-X-SerP, which may well be available as a non-allergic food ingredient having an adjuvant activity for mucosal IgA responses. |
| Databáze: | OpenAIRE |
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