Prenatal binge-like alcohol exposure alters brain and systemic responses to reach sodium and water balance
Autor: | Juan Carlos Molina, J.L. Amigone, Andrea Godino, Paula Abate, Laura Vivas |
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Rok vydání: | 2015 |
Předmět: |
PRENATAL ETHANOL EXPOSURE
Male Vasopressin medicine.medical_specialty CIENCIAS MÉDICAS Y DE LA SALUD media_common.quotation_subject Inmunología Drinking Behavior Binge Drinking Electrolytes Random Allocation Dorsal raphe nucleus SEROTONERGIC NEURONS Extended amygdala Pregnancy Internal medicine Animals Medicine Rats Wistar 5-HT receptor SODIUM BALANCE media_common Neurons business.industry General Neuroscience Solitary tract Brain Sodium Dietary Appetite Water-Electrolyte Balance Immunohistochemistry Medicina Básica Disease Models Animal Endocrinology Prenatal Exposure Delayed Effects VASOPRESSINERGIC NEURONS Magnocellular cell Female Serotonin business |
Zdroj: | Neuroscience. 311:92-104 |
ISSN: | 0306-4522 |
Popis: | The aim of the present work is to analyze how prenatal binge-like ethanol exposure to a moderate dose (2.0 g/kg; group Pre-EtOH) during gestational days (GD) 17-20 affects hydroelectrolyte regulatory responses. This type of exposure has been observed to increase ethanol consumption during adolescence (postnatal day 30-32). In this study we analyzed basal brain neural activity and basal-induced sodium appetite (SA) and renal response stimulated by sodium depletion (SD) as well as voluntary ethanol consumption as a function of vehicle or ethanol during late pregnancy. In adolescent offspring, SD was induced by furosemide and a low-sodium diet treatment (FURO + LSD). Other animals were analyzed in terms of immunohistochemical detection of Fra-like (Fra-LI-ir) protein and serotonin (5HT) and/or vasopressin (AVP). The Pre-EtOH group exhibited heightened voluntary ethanol intake and a reduction in sodium and water intake induced by SD relative to controls. Basal Na and K concentrations in urine were also reduced in Pre-EtOH animals while the induced renal response after FURO treatment was similar across prenatal treatments. However, the correlation between urine volume and water intake induced by FURO significantly varied across these treatments. At the brain level of analysis, the number of basal Fra-LI-ir was significantly increased in AVP magnocellular neurons of the paraventricular nucleus (PVN) and in 5HT neurons in the dorsal raphe nucleus (DRN) in Pre-EtOH pups. In the experimental group, we also observed a significant increase in Fra-LI along the nucleus of the solitary tract (NTS) and in the central extended amygdala nuclei. In summary, moderate Pre-EtOH exposure produces long-lasting changes in brain organization, affecting basal activity of central extended amygdala nuclei, AVP neurons and the inhibitory areas of SA such as the NTS and the 5HT-DRN. These changes possibly modulate the above described variations in basal-induced drinking behaviors and renal regulatory responses. Fil: Godino, Maria Andrea del Milagro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Abate, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Nacional de Córdoba; Argentina Fil: Amigone, J.L.. Hospital Privado Centro Medico de Cordoba; Argentina Fil: Vivas, Laura Marta. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Molina, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Nacional de Córdoba; Argentina |
Databáze: | OpenAIRE |
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