Dual-detector-post-column reactor system for the detection of isoenzymes separated by high-performance liquid chromatography
| Autor: | Timothy D. Schlabach, Joe A. Fulton, E. Clifford Toren, James E. Kerl |
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| Rok vydání: | 1979 |
| Předmět: |
Accuracy and precision
Chromatography Resolution (mass spectrometry) Dynamic range Physics::Instrumentation and Detectors Chemistry Coefficient of variation Organic Chemistry Detector Analytical chemistry General Medicine Biochemistry High-performance liquid chromatography Lactate dehydrogenase isoenzymes Orders of magnitude (mass) Analytical Chemistry Column (typography) Experimental system Dual detector Electromagnetic coil Theoretical plate Dispersion (chemistry) Column (data store) |
| Zdroj: | Journal of Chromatography A. 175:269-281 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(00)89433-9 |
| Popis: | We describe a dual-detector-post-column chromatographic reaction detector system that corrects for substances present in biological samples that interfere with the measurement of isoenzymes separated on a chromatographic column. The response observed at the detector in front of the reaction coil is mathematically dispersed, time transformed and subtracted from the detector behind the coil to produce a blank corrected chromatogram. The same computer program calculates peak areas and other chromatographic parameters such as height equivalent to a theoretical plate and retention time. In addition, we have evaluated the dispersion effects caused by various changes in our experimental system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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