Structure/function analysis of the Ala116← Lys121region of endonuclease V by random targeted mutagenesis

Lys121 using a random targeted mutagenesis procedure developed for this study. The results presented here suggest an important role for this region in the stabilization of the thymine dimer-containing substrate. These mutants also confirm a direct correlation between survival and both DNA binding and pyrimidine dimer-DNA glycosylase activity. No such correlation exists between survival and AP lyase activity. The results are consistent with the recently published X-ray crystal structure. -->

ISSN:	1362-4962 0305-1048
DOI:	10.1093/nar/21.3.727
Přístupová URL adresa:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7985ae68a945ffbb637b6d394b59af7 https://doi.org/10.1093/nar/21.3.727
Rights:	OPEN
Přírůstkové číslo:	edsair.doi.dedup.....c7985ae68a945ffbb637b6d394b59af7
Autor:	Anthony P. Green, Earl E. Henderson, Jon K. deRiel
Rok vydání:	1993
Předmět:	Pyrimidine Molecular Sequence Data Mutant Pyrimidine dimer Biology Deoxyribonuclease (Pyrimidine Dimer) Structure-Activity Relationship chemistry.chemical_compound Escherichia coli Genetics Bacteriophage T4 Cloning Molecular Gene Alanine Endodeoxyribonucleases Base Sequence Lysine Molecular biology Peptide Fragments Thymine chemistry Biochemistry Mutagenesis DNA glycosylase DNA Viral DNA
Zdroj:	Nucleic Acids Research. 21:727-732
ISSN:	1362-4962 0305-1048
DOI:	10.1093/nar/21.3.727
Popis:	Endonuclease V is the product of the denV gene of bacteriophage T4 and is responsible for the recognition and repair of pyrimidine dimers due to UV irradiation of DNA. This is accomplished by a two-step mechanism involving incision at the site of the lesion followed by cleavage of the phosphate backbone. In order to better understand this molecule, and to validate our new mutagenesis procedure, we have constructed a series of random mutations within the region Ala116-->Lys121 using a random targeted mutagenesis procedure developed for this study. The results presented here suggest an important role for this region in the stabilization of the thymine dimer-containing substrate. These mutants also confirm a direct correlation between survival and both DNA binding and pyrimidine dimer-DNA glycosylase activity. No such correlation exists between survival and AP lyase activity. The results are consistent with the recently published X-ray crystal structure.
Databáze:	OpenAIRE
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