A novel cypovirus found in a betabaculovirus co-infection context contains a poxvirus immune nuclease (poxin)-related gene
| Autor: | Bergmann Morais Ribeiro, Leonardo Assis da Silva, Daniel M. P. Ardisson-Araújo, Brenda R. de Camargo, Marlinda Lobo de Souza |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine food.ingredient Hypothetical protein Context (language use) Granulovirus Genome Viral Occlusion Bodies Viral Moths Reoviridae 010603 evolutionary biology 01 natural sciences Lepidoptera genitalia 03 medical and health sciences food Virology Betabaculovirus Complementary DNA Animals Cyclic GMP Phylogeny Genetics Deoxyribonucleases biology Phylogenetic tree Erinnyis ello Coinfection Poxviridae biology.organism_classification Lepidoptera 030104 developmental biology Larva Cypovirus Brazil |
| Zdroj: | The Journal of general virology. 101(6) |
| ISSN: | 1465-2099 |
| Popis: | The cassava hornworm Erinnyis ello ello (Lepidoptera: Sphingidae) is an important pest in Brazil. This insect feeds on host plants of several species, especially Manihot esculenta (cassava) and Hevia brasiliensis (rubber tree). Cassava hornworm outbreaks are quite common in Brazil and can cause great impact over crop production. Granulare and polyhedral-shaped occlusion bodies (OBs) were observed in extracts of dead E. ello larvae from rubber-tree plantations by light and scanning electron microscopy (SEM), suggesting a mixed infection. The polyhedral-shaped OB surface revealed indentations that resemble those found in cypovirus polyhedra. After OB nucleic acid extraction followed by cDNA production and Illumina deep-sequencing analysis, the results confirmed for the presence of a putative novel cypovirus that carries ten segments and also a betabaculovirus (Erinnyis ello granulovirus, ErelGV). Phylogenetic analysis of the predicted segment 1-enconded RdRP showed that the new cypovirus isolate is closely related to a member of species Cypovirus 2, which was isolated from Inachis io (Lepidoptera: Nymphalidae). Therefore, we named this new isolate Erinnyis ello cypovirus 2 (ErelCPV-2). Genome in silico analyses showed that ErelCPV-2 segment 8 (S8) has a predicted amino acid identity of 35.82 % to a hypothetical protein of betabaculoviruses. This putative protein has a cGAMP-specific nuclease domain related to the poxvirus immune nucleases (poxins) from the 2′,3′-cGAMP-degrading enzyme family. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|