Colorimetric and fluorescent determination of sulfide and sulfite with kinetic discrimination
| Autor: | Haiyu Tian, Junhong Qian, Xiaoliang Pei, Albert M. Brouwer, Weibing Zhang |
|---|---|
| Přispěvatelé: | Spectroscopy and Photonic Materials (HIMS, FNWI) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Ketone
Fluorophore Quinolizidines Sulfide Pyridinium Compounds Inorganic chemistry Sulfides Photochemistry Biochemistry Analytical Chemistry Cell Line chemistry.chemical_compound Mice Sulfite Coumarins Electrochemistry Environmental Chemistry Animals Sulfites Colorimetry Spectroscopy Fluorescent Dyes chemistry.chemical_classification Fluorescence eye diseases chemistry Microscopy Fluorescence Pyridinium |
| Zdroj: | Analyst, 139(20), 5290-5296. Royal Society of Chemistry |
| ISSN: | 0003-2654 |
| Popis: | Two fluorescent probes, m-PSP and p-PSP, for sulfite and/or sulfide were constructed by connecting a pyridinium ion to a coumarin fluorophore through an alpha,beta-unsaturated ketone. The presence of the pyridinium salt promoted the nucleophilic addition of sulfite and sulfide to the alpha,beta-unsaturated ketone, which could be visualized by dramatic changes in the solution's color and fluorescence intensity. Both probes exhibit good selectivity (the selectivity coefficients toward major interferences are less than 0.07) and high sensitivity for sulfite and sulfide over biothiols and other potential analytes. The detection limits of m-PSP for the analysis of sulfite and sulfide are calculated to 8.5 x 10(-7) M and 2.7 x 10(-7) M, respectively. Living cell imaging results indicate that both probes can be applied in biological systems. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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