Modulatory effects of melatonin and vitamin C on oxidative stress-mediated haemolytic anaemia and associated cardiovascular dysfunctions in rats

Autor: Temidayo Olutayo Omobowale, Temitayo Olabisi Ajibade, Ademola Adetokunbo Oyagbemi, F. O. Olayemi, Ebunoluwa Racheal Asenuga, Ladoke A. Durotoye
Rok vydání: 2015
Předmět:
0301 basic medicine
Male
medicine.medical_specialty
Anemia
Hemolytic
Antioxidant
Erythrocytes
medicine.medical_treatment
Blood Pressure
Ascorbic Acid
medicine.disease_cause
Kidney
Antioxidants
03 medical and health sciences
chemistry.chemical_compound
Hemoglobins
0302 clinical medicine
Internal medicine
Malondialdehyde
medicine
Animals
Rats
Wistar
Melatonin
chemistry.chemical_classification
Glutathione Peroxidase
Vitamin C
business.industry
Superoxide Dismutase
Glutathione peroxidase
Myocardium
Heart
Hydrogen Peroxide
Vitamins
Ascorbic acid
Catalase
Glutathione
Phenylhydrazines
Oxidative Stress
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Blood pressure
Complementary and alternative medicine
chemistry
030220 oncology & carcinogenesis
Hypertension
business
Oxidative stress
Zdroj: Journal of complementaryintegrative medicine. 14(1)
ISSN: 1553-3840
Popis: Background Phenylhydrazine (PHE) in experimental animal models has been widely reported to cause haemolytic anaemia, via the induction of oxidative stress and thus causing deleterious cardiovascular complications. Hence, this study was designed to evaluate the possible modulatory role of melatonin (MLT) or vitamin C when co-administered with PHE. Methods Anaemia was established with PHE administration. MLT or vitamin C was co-administered with PHE. Haematological parameters, markers of oxidative stress, enzymic and non-enzymic antioxidants, blood pressure and electrocardiograms were assessed. Results PHE administration led to a significant (p2O2) generated in cardiac, renal and red blood cell (RBC) lysates. PHE also significantly reduced the activity of glutathione peroxidase (GPx), superoxide dismutase (SOD) and reduced glutathione (GSH) contents, respectively. The RBC counts, haemoglobin (Hb) concentration and packed cell volume (PCV) were also significantly reduced following the administration of PHE. Furthermore, the systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean arterial blood pressure (MABP) increased significantly in rats administered PHE alone. Similarly, PHE administration led to a significant drop in heart rate but prolonged QRS, QT and QTc interval. Pathology of the heart and kidney was also observed in PHE treated group. However, treatment with MLT and vitamin C improved enzymic and non-enzymic antioxidant system together with the restoration of SBP, DBP and MABP to near normal. The architectural anarchy observed in the heart and kidney of PHE administered rats was reversed to some extent. Conclusions Hence, MLT and vitamin C could be employed as therapeutic targets in various cardiovascular diseases and its complications.
Databáze: OpenAIRE
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