Electrophoretic Properties and Specificity of Human Gastric Lipase
| Autor: | Zdzislaw Szafran, Anna Wanicka, Szafran H, Tadeusz Popiela |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
chemistry.chemical_classification
Gastric Juice Duodenum Chemistry digestive oral and skin physiology Lipase In Vitro Techniques Biochemistry Substrate Specificity Staining Stomach body Electrophoresis Enzyme Gastric Mucosa Pyloric Antrum Humans Electrophoresis Polyacrylamide Gel Gastric lipase Zymography Intestinal Mucosa Polyacrylamide gel electrophoresis Antrum |
| Zdroj: | Enzyme. 23:187-193 |
| ISSN: | 2504-2564 0013-9432 |
| DOI: | 10.1159/000458575 |
| Popis: | Electrophoretic separation of gastric juice and the homogenate of stomach body mucosa on polyacrylamide gel followed by staining for esterolytic activity resulted in a characteristic zymogram composed of five activity bands located in the cathodal part of the electropherogram. This pattern was lacking in zymograms obtained for the homogenates of antral and duodenal mucosa. Lipolytic activity was confined to the same area of the gel where the five band pattern of esterolytic activity was localized. It was concluded that gastric juice and homogenate of stomach body mucosa contain one esterolytic enzyme of wide specificity, known as gastric lipase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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