Characterization of Proteins Involved in Chloroplast Targeting Disturbed by Rice Stripe Virus by Novel Protoplast–Chloroplast Proteomics
| Autor: | Jingjing Xu, Xijiao Song, Lin Lin, Zhuo Chen, Hongying Zheng, Jinping Zhao, Jianping Chen, Yuwen Lu, Zhongjing Zhou, Jiejun Peng, Fei Yan, Binghua Chen, Weijun Cui, Zhiping Deng |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Proteomics
0106 biological sciences 0301 basic medicine Chloroplasts Proteome viruses Nicotiana benthamiana medicine.disease_cause 01 natural sciences Chloroplast membrane lcsh:Chemistry rice stripe virus Tandem Mass Spectrometry Protein targeting lcsh:QH301-705.5 Spectroscopy Plant Proteins Protoplasts food and beverages General Medicine chloroplast proteomics Computer Science Applications Cell biology Chloroplast Phenotype plant–virus interaction nuclear/nucleus-encoded chloroplast-related protein Biology Article Catalysis Inorganic Chemistry 03 medical and health sciences medicine Physical and Theoretical Chemistry Molecular Biology Plant Diseases fungi Organic Chemistry Computational Biology Oryza Rice stripe virus biology.organism_classification chloroplast targeting Gene Ontology 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Chloroplast Proteins Chromatography Liquid 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences Volume 20 Issue 2 International Journal of Molecular Sciences, Vol 20, Iss 2, p 253 (2019) |
| ISSN: | 1422-0067 |
| Popis: | Rice stripe virus (RSV) is one of the most devastating viral pathogens in rice and can also cause the general chlorosis symptom in Nicotiana benthamiana plants. The chloroplast changes associated with chlorosis symptom suggest that RSV interrupts normal chloroplast functions. Although the change of proteins of the whole cell or inside the chloroplast in response to RSV infection have been revealed by proteomics, the mechanisms resulted in chloroplast-related symptoms and the crucial factors remain to be elucidated. RSV infection caused the malformation of chloroplast structure and a global reduction of chloroplast membrane protein complexes in N. benthamiana plants. Here, both the protoplast proteome and the chloroplast proteome were acquired simultaneously upon RSV infection, and the proteins in each fraction were analyzed. In the protoplasts, 1128 proteins were identified, among which 494 proteins presented significant changes during RSV meanwhile, 659 proteins were identified from the chloroplasts, and 279 of these chloroplast proteins presented significant change. According to the label-free LC&ndash MS/MS data, 66 nucleus-encoded chloroplast-related proteins (ChRPs), which only reduced in chloroplast but not in the whole protoplast, were identified, indicating that these nuclear-encoded ChRPswere not transported to chloroplasts during RSV infection. Gene ontology (GO) enrichment analysis confirmed that RSV infection changed the biological process of protein targeting to chloroplast, where 3 crucial ChRPs (K4CSN4, K4CR23, and K4BXN9) were involved in the regulation of protein targeting into chloroplast. In addition to these 3 proteins, 41 among the 63 candidate proteins were characterized to have chloroplast transit peptides. These results indicated that RSV infection changed the biological process of protein targeting into chloroplast and the location of ChRPs through crucial protein factors, which illuminated a new layer of RSV&ndash host interaction that might contribute to the symptom development. |
| Databáze: | OpenAIRE |
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