A dynamic-signaling-team model for chemotaxis receptors in Escherichia coli
| Autor: | Victor Sourjik, Ned S. Wingreen, Clinton H Hansen |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Multidisciplinary
Chemoreceptor Chemotactic Factors Protein Conformation Chemotaxis Escherichia coli Proteins Receptors Cell Surface Cooperativity Methylation Biological Sciences Biology medicine.disease_cause Models Biological Protein structure Biochemistry Escherichia coli medicine Biophysics Signal transduction Receptor Algorithms Signal Transduction |
| Zdroj: | Proc Natl Acad Sci U S A |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1005017107 |
| Popis: | The chemotaxis system of Escherichia coli is sensitive to small relative changes in ambient chemoattractant concentrations over a broad range. Interactions among receptors are crucial to this sensitivity, as is precise adaptation, the return of chemoreceptor activity to prestimulus levels in a constant chemoeffector environment through methylation and demethylation of receptors. Signal integration and cooperativity have been attributed to strongly coupled, mixed teams of receptors, but receptors become individually methylated according to their ligand occupancy states. Here, we present a model of dynamic signaling teams that reconciles strong coupling among receptors with receptor-specific methylation. Receptor trimers of dimers couple to form a honeycomb lattice, consistent with cryo-electron microscopy (cryoEM) tomography, within which the boundaries of signaling teams change rapidly. Our model helps explain the inferred increase in signaling team size with receptor modification, and indicates that active trimers couple more strongly than inactive trimers. |
| Databáze: | OpenAIRE |
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