Individual neuronal subtypes control initial myelin sheath growth and stabilization
| Autor: | Madeline R. Martell, Jacob H. Hines, Samantha T. Maas, Anthony J. Treichel, Silas Bergen, Amanda J. Kaiser, James R. Gronseth, Heather N. Nelson, Erin N. Eggum, Allie G. Trudel |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Embryo Nonmammalian Transgene Central nervous system Biology lcsh:RC346-429 Animals Genetically Modified Myelination Myelin sheath 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience Genes Reporter Glia medicine Animals Axon Process (anatomy) lcsh:Neurology. Diseases of the nervous system Zebrafish Spinal cord Oligodendrocyte Embryonic stem cell Axons Cell biology Oligodendroglia 030104 developmental biology medicine.anatomical_structure Spinal Cord nervous system Larva Developmental biology 030217 neurology & neurosurgery Research Article |
| Zdroj: | Neural Development Neural Development, Vol 15, Iss 1, Pp 1-16 (2020) |
| ISSN: | 1749-8104 |
| DOI: | 10.1186/s13064-020-00149-3 |
| Popis: | Background In the developing central nervous system, pre-myelinating oligodendrocytes sample candidate nerve axons by extending and retracting process extensions. Some contacts stabilize, leading to the initiation of axon wrapping, nascent myelin sheath formation, concentric wrapping and sheath elongation, and sheath stabilization or pruning by oligodendrocytes. Although axonal signals influence the overall process of myelination, the precise oligodendrocyte behaviors that require signaling from axons are not completely understood. In this study, we investigated whether oligodendrocyte behaviors during the early events of myelination are mediated by an oligodendrocyte-intrinsic myelination program or are over-ridden by axonal factors. Methods To address this, we utilized in vivo time-lapse imaging in embryonic and larval zebrafish spinal cord during the initial hours and days of axon wrapping and myelination. Transgenic reporter lines marked individual axon subtypes or oligodendrocyte membranes. Results In the larval zebrafish spinal cord, individual axon subtypes supported distinct nascent sheath growth rates and stabilization frequencies. Oligodendrocytes ensheathed individual axon subtypes at different rates during a two-day period after initial axon wrapping. When descending reticulospinal axons were ablated, local spinal axons supported a constant ensheathment rate despite the increased ratio of oligodendrocytes to target axons. Conclusion We conclude that properties of individual axon subtypes instruct oligodendrocyte behaviors during initial stages of myelination by differentially controlling nascent sheath growth and stabilization. |
| Databáze: | OpenAIRE |
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