Mast Cells Are Abundant in Primary Cutaneous T-Cell Lymphomas: Results from a Computer-Aided Quantitative Immunohistological Study
| Autor: | Georg E. Steiner, Franz Trautinger, Friedrich Erhart, J. Moser, Johanna Eder, Radu Rogojanu, Alexander M. Dohnal, Waltraud Jerney, Melitta Kitzwögerer |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Pathology Skin Neoplasms lcsh:Medicine Cell Degranulation Hematologic Cancers and Related Disorders White Blood Cells 0302 clinical medicine Animal Cells hemic and lymphatic diseases Image Processing Computer-Assisted Medicine and Health Sciences Mast Cells lcsh:Science Connective Tissue Cells Skin Aged 80 and over Multidisciplinary T Cells Fungal Diseases Hematology Dermis Middle Aged Prognosis Mast cell Immunohistochemistry Lymphoma T-Cell Cutaneous 3. Good health Infectious Diseases medicine.anatomical_structure Oncology Connective Tissue 030220 oncology & carcinogenesis Female Lymphomas Cellular Types Anatomy Integumentary System Research Article Adult Cell Physiology medicine.medical_specialty Adolescent Immune Cells T cell Immunology Tryptase Dermatology Biology Skin Diseases Young Adult 03 medical and health sciences Mycosis Fungoides Diagnostic Medicine medicine Humans Aged Tumor microenvironment Blood Cells lcsh:R Cancers and Neoplasms Biology and Life Sciences Cell Biology medicine.disease Lymphoma Biological Tissue 030104 developmental biology Case-Control Studies biology.protein lcsh:Q Bone marrow |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 11, p e0163661 (2016) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0163661 |
| Popis: | Background Mast cells (MC) are bone marrow derived haematopoetic cells playing a crucial role not only in immune response but also in the tumor microenvironment with protumorigenic and antitumorigenic functions. The role of MC in primary cutaneous T-cell lymphomas (CTCL), a heterogeneous group of non-Hodgkin lymphomas with initial presentation in the skin, is largely unknown. Objective To gain more accurate information about presence, number, distribution and state of activation (degranulated vs. non-degranulated) of MC in CTCL variants and clinical stages. Materials and Methods We established a novel computer-aided tissue analysis method on digitized skin sections. Immunohistochemistry with an anti-MC tryptase antibody was performed on 34 biopsies of different CTCL subtypes and on control skin samples. An algorithm for the automatic detection of the epidermis and of cell density based CTCL areas was developed. Cells were stratified as being within the CTCL infiltrate, in P1 (a surrounding area 0–30 μm away from CTCL), or in P2 (30–60 μm away from CTCL) area. Results We found high MC counts within CTCL infiltrates and P1 and a decreased MC number in the surrounding dermis P2. Higher MC numbers were found in MF compared to all other CTCL subgroups. Regarding different stages of MF, we found significantly higher mast cell counts in stages IA and IB than in stages IIA and IIB. Regarding MC densities, we found a higher density of MC in MF compared to all other CTCL subgroups. More MC were non-degranulated than degranulated. Conclusion Here for the first time an automated method for MC analysis on tissue sections and its use in CTCL is described. Eliminating error from investigator bias, the method allows for precise cell identification and counting. Our results provide new insights on MC distribution in CTCL reappraising their role in the pathophysiology of CTCL. |
| Databáze: | OpenAIRE |
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