Use of commercially available rabbit monoclonal antibodies for immunofluorescence double staining
| Autor: | Michael Bzorek, Bodil Laub Petersen, Inger Stamp, Lisbeth Frederiksen |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Histology
medicine.drug_class Fluorescent Antibody Technique Monoclonal antibody Immunofluorescence Pathology and Forensic Medicine Mice Antigen medicine Animals Humans CD20 Paraffin Embedding medicine.diagnostic_test biology Staining and Labeling Chemistry Goats Antibodies Monoclonal Precursor Cell Lymphoblastic Leukemia-Lymphoma CD79A Molecular biology Medical Laboratory Technology Polyclonal antibodies biology.protein Immunohistochemistry Rabbits CD5 |
| Zdroj: | Applied immunohistochemistrymolecular morphology : AIMM. 16(4) |
| ISSN: | 1533-4058 |
| Popis: | Immunohistochemistry, that is, the use of polyclonal and monoclonal antibodies to detect cell and tissue antigens at a microscopical level is a powerful tool for both research and diagnostic purposes. Especially in the field of hematologic disease, there is often a need to detect several antigens synchronously, and we report here a fast and easy technique for demonstrating more than 1 antigen in 1 slide using immunofluorescence. We have used commercially available rabbit monoclonal antibodies (Cyclin D1, CD3, CD5, CD23, etc.) paired with mouse monoclonal antibodies (CD7, CD20, CD79a, Pax-5, etc.) for double immunofluorescence labeling on paraffin-embedded tissue sections. Commercially available rabbit monoclonal antibodies in combination with mouse monoclonal antibodies proved useful in double immunofluorescence labeling on paraffin-embedded tissue, and all combinations used yielded excellent results. |
| Databáze: | OpenAIRE |
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