A simple method for in vitro preparation of natural killer cells from cord blood
| Autor: | Yu Xia Zhao, Li Yun Bai, Xiao Yun Wu, Yong Xu Mu, Hui Jiang, Hong Jing Bao, Yun Hong Wang, Xiao Lei Qi, Zhi Jie Ma, Bing Yao Li |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Expansion
lcsh:Biotechnology Cytotoxicity medicine.medical_treatment CD16 Biology Zoledronic Acid Peripheral blood mononuclear cell 03 medical and health sciences 0302 clinical medicine lcsh:TP248.13-248.65 medicine Humans 030304 developmental biology 0303 health sciences Methodology Article Cord blood Immunotherapy Cell sorting Fetal Blood Flow Cytometry NKG2D In vitro Cell biology Killer Cells Natural 030220 oncology & carcinogenesis Leukocytes Mononuclear Natural killer cells Interleukin-2 K562 Cells Biotechnology K562 cells |
| Zdroj: | BMC Biotechnology BMC Biotechnology, Vol 19, Iss 1, Pp 1-8 (2019) |
| ISSN: | 1472-6750 |
| Popis: | Background Cord Blood (CB) has been considered a promising source of natural killer (NK) cells for cellular immunotherapy. However, it is difficult to expand the large numbers of highly pure NK cells from CB without cell sorting and feeder cells/multiple cytokines. In this study, we try to develop a simple, safe and economical method for ex vivo expansion and purification of NK cells from CB without cell sorting and feeder cells/multiple cytokines. Results The large numbers (mean: 1.59 × 1010) of highly pure (≥90%) NK cells from CB could be obtained through interleukin-2, group A streptococcus and zoledronate stimulation of mononuclear cells using the 21-day culture approach. When compared to resting NK cells, expanded NK cells were a higher expression of activating receptors CD16, NKG2D, NKp30, NKp44, NKp46 and activating markers CD62L and CD69, while the inhibitory receptors, CD158a and CD158b remained largely unchanged. In addition, these cells showed a higher concentration of IFN-γ, TNF-α and GM-CSF secretion and cytotoxicity to K562 cells and acute myeloid leukemia targets than resting NK cells. Conclusion We develop a simple, safe and economical method to obtain high yield, purity, and functionality NK cells from CB without cell sorting and feeder cells/multiple cytokines. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink