Prevalence of heterophilic antibodies in serum samples from horses in an equine hospital, and elimination of interference using chicken IgY
| Autor: | Daniel Bergman, Bo Dong, Bodil Ström Holst |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Serum Population Clinical science Immunoglobulins Antibodies Heterophile Enzyme-Linked Immunosorbent Assay Horse 03 medical and health sciences Mice 0302 clinical medicine Prevalence Medicine Animals Horses education Heterophilic antibodies Immunoassay education.field_of_study CATS lcsh:Veterinary medicine General Veterinary biology business.industry Research General Medicine Clinical Science Serum samples 030104 developmental biology 030220 oncology & carcinogenesis Immunoglobulin G Immunology biology.protein lcsh:SF600-1100 Horse Diseases ELISA Antibody Horse serum business Interference Chickens |
| Zdroj: | Acta Veterinaria Scandinavica, Vol 63, Iss 1, Pp 1-6 (2021) Acta Veterinaria Scandinavica |
| ISSN: | 1751-0147 |
| Popis: | Background Heterophilic antibodies in serum and plasma can interfere with mammalian antibodies in immunoassays and result in false test results, usually false positive. Although studies screening for heterophilic antibodies as well as elimination studies have been conducted in dogs and cats, knowledge of the presence of heterophilic antibodies in other species in veterinary medicine is limited. In this study, a 2-site sandwich-type interference assay that detects anti-mouse antibodies was used to detect heterophilic antibodies in a population of horses treated in an animal hospital. Results A total of 194 serum samples from 127 individual horses were analyzed. There were 11/127 (8.7%) interference-positive horses, and these were analyzed in an assay exchanging the capture mouse IgG with chicken IgY. The positive samples were negative in the chicken IgY assay, indicating elimination of a possible interference, with the chicken-based assay. Four interference-positive samples were from geldings, and anti-Müllerian hormone (AMH) was analyzed from these samples. AMH concentrations were negative in these samples as expected in geldings, indicating that the heterophilic antibodies did not cause interference in the AMH assay. Conclusion The present study shows that there are heterophilic antibodies in horse serum samples like in samples from humans, dogs, and cats. The use of chicken-based reagents, such as chicken IgY, which do not cross-react with mammalian IgG, eliminates the effects of interfering antibodies in the samples. Equine heterophilic antibodies do not necessarily cause interference in commercial immunoassays. |
| Databáze: | OpenAIRE |
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