Immunohistochemical Mapping of TRK-Fused Gene Products in the Rat Brainstem
| Autor: | Ikuo Tooyama, Chiaki Masuda, Shigeko Takeuchi, Hisae Maebayashi |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Cerebellum
Pathology medicine.medical_specialty Histology Physiology brain TFG Biochemistry Pathology and Forensic Medicine oncogene Medicine business.industry Gigantocellular reticular nucleus Regular Article Cell Biology Spinal cord neuron Pons Cell biology medicine.anatomical_structure Dorsal motor nucleus nervous system immunohistochemistry Neuron Brainstem business Raphe nuclei |
| Zdroj: | Acta Histochemica et Cytochemica |
| ISSN: | 1347-5800 0044-5991 |
| DOI: | 10.1267/ahc.11051 |
| Popis: | The TRK-fused gene (TFG in human, Tfg in rat) was originally identified in human papillary thyroid cancer as a chimeric form of the NTRK1 gene. It was since reported that the gene product (TFG) plays a role in regulating phosphotyrosine-specific phosphatase-1 activity. As shown in the accompanying paper, we produced an antibody to rat TFG and used it to localize TFG to selected neurons in specific regions. In the present study, we mapped the TFG-positive neurons in the brainstem, cerebellum, and spinal cord of rats. In the brainstem, neurons intensely positive for TFG were distributed in the raphe nuclei, the gigantocellular reticular nucleus, the reticulotegmental nucleus of the pons, and some cranial nerve nuclei such as the trigeminal nuclei, the vestibulocochlear nuclei, and the dorsal motor nucleus of the vagus. Purkinje cells in the cerebellum and motor neurons in the spinal anterior horn were also positive for TFG. These results provide fundamental data for studying the functions of TFG in the brain. |
| Databáze: | OpenAIRE |
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