Ultrasensitive electrochemical detection of poly (ADP-ribose) polymerase-1 via polyaniline deposition
| Autor: | Yuanjian Liu, Yong Liu, Jiahui Fan, Wei Wei, Li Shangguan, Yuanqing Wei, Songqin Liu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Poly Adenosine Diphosphate Ribose
Poly ADP ribose polymerase Drug Evaluation Preclinical Poly (ADP-Ribose) Polymerase-1 02 engineering and technology Nicotinamide adenine dinucleotide 010402 general chemistry Electrochemistry 01 natural sciences Horseradish peroxidase Analytical Chemistry chemistry.chemical_compound Polyaniline Humans Enzyme Inhibitors Enzyme Assays Detection limit Aniline Compounds biology Electrochemical Techniques 021001 nanoscience & nanotechnology Combinatorial chemistry 0104 chemical sciences Adenosine diphosphate chemistry biology.protein NAD+ kinase 0210 nano-technology |
| Zdroj: | Talanta. 180:127-132 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2017.11.072 |
| Popis: | Recent findings have thrust poly ADP (ADP: adenosine diphosphate)-ribose polymerase-1 (PARP-1) into the limelight as potential chemotherapeutic target because it is closely related to the development of tumor. So, studies on its detection and inhibitors evaluation have attracted more attention. It is interesting that poly (ADP-ribose) (PAR), the catalytic product of PARP-1 in the existence of nicotinamide adenine dinucleotide (NAD+), possess twice charge density of DNA strands. PAR contain 200 units, i.e., about 400bp bases, and multiple branched strands. So, plentiful negative charges on PAR supplied exquisite environment for PANI deposition, which was triggered by horseradish peroxidase (HRP). Because of the unique electrochemical property of PANI, ultrasensitive electrochemical detection of PARP-1 was proposed. Under optimum conditions, DPV intensity linearly increased with the increment of PARP-1 in the range of 0.005-1.0 U. The detection limit was 0.002 U, which was comparable or more sensitive than that obtained from previously reported strategies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect