Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics
| Autor: | Jongnam Kim, Jongsoo Chang, Jaeyong Song, Seongwon Seo, Gui-Seck Bae, Moon Baek Chang, Chang-Hyun Kim, Eun Joong Kim |
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| Rok vydání: | 2018 |
| Předmět: |
Rumen Microbiota
animal structures biology Chemistry Denaturing Gradient Gel Electrophoresis Microorganism food and beverages Fasting Prevotella ruminicola biology.organism_classification Prevotella albensis Article Ruminant Nutrition and Forage Utilization Rumen In vitro In vivo Holstein Steers Animal Science and Zoology Dry matter Fermentation Food science Temperature gradient gel electrophoresis Food Science |
| Zdroj: | Asian-Australasian Journal of Animal Sciences |
| ISSN: | 1011-2367 |
| Popis: | OBJECTIVE Fasting may lead to changes in the microbiota and activity in the rumen. In the present study, the effects of fasting on rumen microbiota and the impact of fasting on in vitro rumen fermentation were evaluated using molecular culture-independent methods. METHODS Three ruminally cannulated Holstein steers were fed rice straw and concentrates. The ruminal fluids were obtained from the same steers 2 h after the morning feeding (control) and 24 h after fasting (fasting). The ruminal fluid was filtrated through four layers of muslin, collected for a culture-independent microbial analysis, and used to determine the in vitro rumen fermentation characteristics. Total DNA was extracted from both control and fasting ruminal fluids. The rumen microbiota was assessed using denaturing gradient gel electrophoresis (DGGE) and quantitative polymerase chain reaction. Microbial activity was evaluated in control and fasting steers at various intervals using in vitro batch culture with rice straw and concentrate at a ratio of 60:40. RESULTS Fasting for 24 h slightly affected the microbiota structure in the rumen as determined by DGGE. Additionally, several microorganisms, including Anaerovibrio lipolytica, Eubacterium ruminantium, Prevotella albensis, Prevotella ruminicola, and Ruminobacter amylophilus, decreased in number after fasting. In addition, using the ruminal fluid as the inoculum after 24 h of fasting, the fermentation characteristics differed from those obtained using non-fasted ruminal fluid. Compared with the control, the fasting showed higher total gas production, ammonia, and microbial protein production (p |
| Databáze: | OpenAIRE |
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