Chitin-deacetylase activity induces appressorium differentiation in the rice blast fungus Magnaporthe oryzae
| Autor: | Hayao Taguchi, Yuko Ohno, Tohru Teraoka, Masahiro Nakajima, Sayaka Murata, Misa Kuroki, Takashi Kamakura, Akihito Nozaka, Kana Okauchi, Ken-ichiro Saitoh, Yuichi Nakajima, Nobukiyo Tanaka, Sho Yoshida, Megumi Narukawa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:Medicine Germ tube Chitin Fungus macromolecular substances medicine.disease_cause 01 natural sciences Article Microbiology Amidohydrolases Cell wall 03 medical and health sciences chemistry.chemical_compound Enzyme activator medicine Amino Acid Sequence lcsh:Science Plant Diseases chemistry.chemical_classification Appressorium Mutation Multidisciplinary biology lcsh:R fungi Genetic Complementation Test food and beverages Oryza biology.organism_classification carbohydrates (lipids) Enzyme Activation Magnaporthe 030104 developmental biology Enzyme chemistry Host-Pathogen Interactions lcsh:Q 010606 plant biology & botany |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-8 (2017) |
| ISSN: | 2045-2322 |
| Popis: | The rice blast fungus Magnaporthe oryzae differentiates a specialized infection structure called an appressorium to invade rice cells. In this report, we show that CBP1, which encodes a chitin-deacetylase, is involved in the induction phase of appressorium differentiation. We demonstrate that the enzymatic activity of Cbp1 is critical for appressorium formation. M. oryzae has six CDA homologues in addition to Cbp1, but none of these are indispensable for appressorium formation. We observed chitosan localization at the fungal cell wall using OGA488. This observation suggests that Cbp1-catalysed conversion of chitin into chitosan occurs at the cell wall of germ tubes during appressorium differentiation by M. oryzae. Taken together, our results provide evidence that the chitin deacetylase activity of Cbp1 is necessary for appressorium formation. |
| Databáze: | OpenAIRE |
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