Role of HSP70 in response to (thermo)radiotherapy: analysis of gene expression in canine osteosarcoma cells by RNA-seq
| Autor: | Mathias S. Weyland, Carla Rohrer Bley, Pauline Thumser-Henner, Giancarlo Russo, Katarzyna J. Nytko |
|---|---|
| Přispěvatelé: | University of Zurich, Nytko, Katarzyna J |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Disease model
animal 10253 Department of Small Animals Vesicular Transport Proteins lcsh:Medicine Bone neoplasm 615: Pharmakologie und Therapeutik Membrane glycoprotein Gene expression Dog Vesicular transport protein Bone cancer Cluster Analysis RNA-Seq 11434 Center for Clinical Studies RNA Small Interfering lcsh:Science Cancer Regulation of gene expression Gene knockdown Osteosarcoma Principal Component Analysis Multidisciplinary Membrane Glycoproteins HSP70 heat-shock protein Transfection Photon Gene Expression Regulation Neoplastic Matrix Metalloproteinase 1 Hyperthermia 610 Medicine & health Bone Neoplasms Biology Canine Osteosarcoma Article Dogs medicine Animals HSP70 Heat-Shock Proteins RNA Messenger Cell Proliferation 1000 Multidisciplinary Photons 11077 Center for Applied Biotechnology and Molecular Medicine Radiotherapy Animal 572: Biochemie Cell growth lcsh:R Hyperthermia Induced medicine.disease Disease Models Animal Cancer research lcsh:Q |
| Zdroj: | Scientific Reports Scientific Reports, 10 (1) Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Pre-treatment of tumors with hyperthermia is often used to increase the efficacy of radiotherapy. One of the main proteins induced in response to hyperthermia is heat shock protein 70 (HSP70). The aim of our study was to investigate up- and down-regulated genes in response to (thermo)radiotherapy in HSP70 proficient and deficient canine osteosarcoma cell line (Abrams), and functional role of HSP70 in the mechanism of thermoradiosensitization. Cells were transfected with negative control siRNA or siRNA targeting HSP70 and treated with hyperthermia (HT), radiotherapy (RT), and thermoradiotherapy (HTRT). RNA sequencing was used to analyze gene expression. Hyperthermia and thermoradiotherapy, but not radiotherapy alone, induced differential gene expression. We identified genes differentially expressed only in HSP70 knockdown (thus HSP70-dependent) cells in response to hyperthermia and thermoradiotherapy. Interestingly, cell proliferation but not clonogenicity and apoptosis/necrosis was affected by the HSP70 knockdown in response to thermoradiotherapy. The results suggest that HSP70 regulates expression of specific genes in response to hyperthermia and thermoradiotherapy. Further investigations into the role of specific genes regulated in a HSP70-dependent manner in response to thermoradiotherapy could pave a way into new, combinatorial treatment options for (canine) osteosarcoma and other cancer types. Scientific Reports, 10 (1) ISSN:2045-2322 |
| Databáze: | OpenAIRE |
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