Uterine and placental specific localization of AQP2 and AQP8 is related with changes of serum progesterone levels in pregnant queens
| Autor: | Teresa Rigau, Maria Montserrat Rivera del Alamo, Lluis Ferré-Dolcet, Meritxell Vendrell, Joan E. Rodríguez-Gil, Marc Yeste |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Placenta
Aquaporin Biology Endometrium Aquaporins Antibodies Andrology 03 medical and health sciences 0302 clinical medicine Food Animals Western blot Pregnancy medicine Animals Tissue Distribution Small Animals reproductive and urinary physiology Progesterone 030219 obstetrics & reproductive medicine Aquaporin 2 medicine.diagnostic_test urogenital system Equine Uterus 0402 animal and dairy science Myometrium Trophoblast 04 agricultural and veterinary sciences medicine.disease 040201 dairy & animal science Immunohistochemistry medicine.anatomical_structure Gene Expression Regulation Cats Animal Science and Zoology Female |
| Zdroj: | Theriogenology. 142 |
| ISSN: | 1879-3231 |
| Popis: | Aquaporins play vital roles in reproductive physiology. This study evaluates the expression and localization dynamics of AQP1, AQP2, AQP3 and AQP8 in the endometrium and placental transference zone during pregnancy in queens by means of immunohistochemistry and Western blot. Animals were distributed into six groups: non-pregnant queens with low levels of serum progesterone (P4), non-pregnant animals with high P4 levels, and queens at 30, 40, 50 and 60 days of pregnancy. All AQPs were present in glandular and luminal epithelia and myometrium. AQP1 was also present in the endometrial endothelia. AQP2, AQP3 and AQP8 were found in trophoblast. In endometrial samples with P4 above 2 ng/mL, AQP2 and AQP8 were distributed across plasma membrane and cytoplasm, whereas progesterone levels under 1 ng/mL kept both AQPs confined to the plasma membrane. Western blot showed no significant changes in AQPs expression among the stages. In conclusion, our results indicate that the distribution of AQP2 and AQP8 in the queen reproductive tract is related to P4 levels. |
| Databáze: | OpenAIRE |
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