A game of hide and seek between avirulence genes AvrLm4‐7 and AvrLm3 in Leptosphaeria maculans
| Autor: | Isabelle Fudal, Thierry Rouxel, Marie-Hélène Balesdent, Clémence Plissonneau, Benedicte Ollivier, Alexandre Degrave, Guillaume Daverdin, Francoise Blaise |
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| Přispěvatelé: | BIOlogie et GEstion des Risques en agriculture (BIOGER), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), CTPS (Projet 'ICOSCOP'), Méta-programme INRA SMaCH (Projet 'K-Masstec'), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Université d'Angers (UA)-AGROCAMPUS OUEST-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chromosomes Artificial Bacterial oilseed rape Physiology durable resistance [SDV]Life Sciences [q-bio] Genes Fungal interaction Plant Science Genes Plant Nucleic Acid Denaturation avirulence Fungal Proteins 03 medical and health sciences Gene mapping Leptosphaeria maculans Ascomycota Amino Acid Sequence next-generation sequencing (NGS) Gene Plant Diseases Genetics Bacterial artificial chromosome Fungal protein Polymorphism Genetic biology Base Sequence Virulence Brassica napus Reproducibility of Results Molecular Sequence Annotation Gene Expression Regulation Bacterial biology.organism_classification Physical Chromosome Mapping Phenotype Complementation 030104 developmental biology Genetic Loci Reference genome Protein Binding |
| Zdroj: | New Phytologist New Phytologist, Wiley, 2016, 209 (4), pp.1613-1624. ⟨10.1111/nph.13736⟩ |
| ISSN: | 0028-646X 1469-8137 |
| DOI: | 10.1111/nph.13736⟩ |
| Popis: | International audience; Extending the durability of plant resistance genes towards fungal pathogens is a major challenge. We identified and investigated the relationship between two avirulence genes of Leptosphaeria maculans, AvrLm3 and AvrLm4‐7. When an isolate possesses both genes, the Rlm3‐mediated resistance of oilseed rape (Brassica napus) is not expressed due to the presence of AvrLm4‐7 but virulent isolates toward Rlm7 recover the AvrLm3 phenotype.Combining genetic and genomic approaches (genetic mapping, RNA‐seq, BAC (bacterial artificial chromosome) clone sequencing and de novo assembly) we cloned AvrLm3, a telomeric avirulence gene of L.maculans. AvrLm3 is located in a gap of the L.maculans reference genome assembly, is surrounded by repeated elements, encodes for a small secreted cysteine‐rich protein and is highly expressed at early infection stages.Complementation and silencing assays validated the masking effect of AvrLm4‐7 on AvrLm3 recognition by Rlm3 and we showed that the presence of AvrLm4‐7 does not impede AvrLm3 expression in planta. Y2H assays suggest the absence of physical interaction between the two avirulence proteins.This unusual interaction is the basis for field experiments aiming to evaluate strategies that increase Rlm7 durability. |
| Databáze: | OpenAIRE |
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