Early nitrogen supplementation stimulates the nodulation and growth of common bean plants inoculated with rhizobium
| Autor: | Luciana Fernandes de Brito, Bárbara Cavalheiro Zoffoli, Rosângela Straliotto, Adelson Paulo Araújo |
|---|---|
| Přispěvatelé: | BÁRBARA CAVALHEIRO ZOFFOLI, UFRRJ, LUCIANA FERNANDES BRITO, NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY, ROSANGELA STRALIOTTO, CNPS, ADELSON PAULO DE ARAÚJO, UFRRJ. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Root nodule
Fertilizante Nitrogenado Inoculação Agriculture (General) Rhizobia S1-972 Fixação de Nitrogênio Nitrogen fixation inoculation Seed inoculation Nitrogen fertilizers biology fungi food and beverages Nitrogenase nitrogen fertilization biology.organism_classification Hydroponics Horticulture phaseolus vulgaris rhizobia nitrogen fixation Shoot Phaseolus Vulgaris Rhizobium Phaseolus Phaseolus vulgaris Agronomy and Crop Science |
| Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA Acta Scientiarum. Agronomy Acta Scientiarum. Agronomy; Vol 43 (2021): Publicação contínua; e55105 Acta Scientiarum. Agronomy; v. 43 (2021): Publicação contínua; e55105 Universidade Estadual de Maringá (UEM) instacron:UEM Acta Scientiarum: Agronomy, Vol 43, Pp e55105-e55105 (2021) Acta Scientiarum. Agronomy, Volume: 43, Article number: e55105, Published: 22 SEP 2021 |
| ISSN: | 1807-8621 1679-9275 |
| Popis: | The initial development of common bean plants (Phaseolus vulgaris L.) relying on symbiotic nitrogen (N) can be limited by delayed active N2 fixation, requiring supplemental N which in turn may inhibit the symbiosis. Five experiments were carried out in hydroponics to identify the initiation of nodulation and nitrogenase activity in common bean cultivars, and the effects of additions of mineral N on plant nodulation and growth. Three experiments evaluated the initial growth of five inoculated bean cultivars in the absence or presence of mineral N, and two experiments evaluated the effect of the moment of mineral N addition until the beginning of reproductive stage. The first root nodules appeared 10 days after plant transplant (DAT) and nitrogenase activity initiated 11 DAT. Cultivars of large seeds had lower initial nodulation and nitrogenase activity than those of small seeds. Inoculated plants showed limited shoot growth that lasted until 21-25 DAT as compared to inoculated plants receiving mineral N. Addition of mineral N reduced nodule mass more intensively than nodule number and more strongly nitrogenase activity. Nitrogen applied until 15 DAT enhanced nodulation and nitrogenase activity without limiting shoot growth, as compared to plants receiving N throughout their growth. Otherwise, plants that received N after 15 DAT had lower nodule mass and nitrogenase activity than plants only inoculated. The results indicate that symbiotic N did not suffice to an adequate growth of common beans and some supplemental N is necessary. This N should be added in the beginning of growth cycle to stimulate plant growth without inhibiting further nodulation and N fixation. The initial development of common bean plants (Phaseolus vulgaris L.) relying on symbiotic nitrogen (N) can be limited by delayed active N2 fixation, requiring supplemental N which in turn may inhibit the symbiosis. Five experiments were carried out in hydroponics to identify the initiation of nodulation and nitrogenase activity in common bean cultivars, and the effects of additions of mineral N on plant nodulation and growth. Three experiments evaluated the initial growth of five inoculated bean cultivars in the absence or presence of mineral N, and two experiments evaluated the effect of the moment of mineral N addition until the beginning of reproductive stage. The first root nodules appeared 10 days after plant transplant (DAT) and nitrogenase activity initiated 11 DAT. Cultivars of large seeds had lower initial nodulation and nitrogenase activity than those of small seeds. Inoculated plants showed limited shoot growth that lasted until 21-25 DAT as compared to inoculated plants receiving mineral N. Addition of mineral N reduced nodule mass more intensively than nodule number and more strongly nitrogenase activity. Nitrogen applied until 15 DAT enhanced nodulation and nitrogenase activity without limiting shoot growth, as compared to plants receiving N throughout their growth. Otherwise, plants that received N after 15 DAT had lower nodule mass and nitrogenase activity than plants only inoculated. The results indicate that symbiotic N did not suffice to an adequate growth of common beans and some supplemental N is necessary. This N should be added in the beginning of growth cycle to stimulate plant growth without inhibiting further nodulation and N fixation. |
| Databáze: | OpenAIRE |
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