HyPer2 imaging reveals temporal and heterogeneous hydrogen peroxide changes in denervated and aged skeletal muscle fibers in vivo
| Autor: | Anne McArdle, Caroline A. Staunton, Natalie Pollock, Malcolm J. Jackson, Aphrodite Vasilaki, Richard Barrett-Jolley, Euan Owen |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Aging Sarcopenia medicine.medical_specialty Nerve Crush Muscle Fibers Skeletal Neuromuscular Junction lcsh:Medicine medicine.disease_cause Peroxide Article Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Internal medicine medicine Animals lcsh:Science Hydrogen peroxide Fluorescent Dyes Denervation Multidisciplinary lcsh:R Skeletal muscle Hydrogen Peroxide Oxidants Mitochondria Experimental models of disease Motor unit Ageing Muscular Atrophy Oxidative Stress 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry Molecular Probes lcsh:Q 030217 neurology & neurosurgery Oxidative stress Ex vivo |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-51035-w |
| Popis: | To determine the role of denervation and motor unit turnover in the age-related increase in skeletal muscle oxidative stress, the hydrogen peroxide (H2O2) specific, genetically-encoded, fluorescent cyto-HyPer2 probe was expressed in mouse anterior tibialis (AT) muscle and compared with ex vivo measurements of mitochondrial oxidant generation. Crush of the peroneal nerve induced increased mitochondrial peroxide generation, measured in permeabilised AT fibers ex vivo and intra vital confocal microscopy of cyto-HyPer2 fluorescence showed increased cytosolic H2O2 in a sub-set (~24%) of individual fibers associated with onset of fiber atrophy. In comparison, mitochondrial peroxide generation was also increased in resting muscle from old (26 month) mice compared with adult (6–8 month) mice, but no age effect on fiber cytosolic H2O2in vivo was seen. Thus ageing is associated with an increased ability of muscle fibers to maintain cytosolic redox homeostasis in the presence of denervation-induced increase in mitochondrial peroxide generation. |
| Databáze: | OpenAIRE |
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