Effect of a two-week treatment with a low dose of 2,2'4,4' 5,5'-hexacholorobiphenyl (PCB153) on tight junction protein expression in ovine choroid plexus during long and short photoperiods
| Autor: | Laurence Dufourny, Christine Lagaraine, Aleksandra Szczepkowska, Jean-Claude Thiéry, Vincent Robert, Janina Skipor |
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| Přispěvatelé: | Institute of Animal Reproduction and Food Research, Polska Akademia Nauk (PAN), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Ministry of Science and Higher Education, Poland, by Grant No. 258/N-INRA/2008/0 and French INRA Grant No. DWM/N19/INRA/2008, Polska Akademia Nauk = Polish Academy of Sciences (PAN), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] Time Factors tight junction Blotting Western Gene Expression Biology Toxicology Occludin photoperiod blood cerebrospinal fluid barrier Tight Junctions 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Cerebrospinal fluid Developmental Neuroscience Internal medicine Blood plasma medicine Animals Tissue Distribution 030304 developmental biology photoperiodism 0303 health sciences Sheep Tight Junction Proteins choroid plexus Tight junction Dose-Response Relationship Drug Polychlorinated Biphenyls ewe Blot Dose–response relationship Endocrinology Choroid plexus Environmental Pollutants Female Seasons 030217 neurology & neurosurgery polychlorinated biphenyl |
| Zdroj: | Neurotoxicology and Teratology Neurotoxicology and Teratology, Elsevier, 2013, 37, pp.63-67. ⟨10.1016/j.ntt.2013.03.061⟩ |
| ISSN: | 0892-0362 |
| DOI: | 10.1016/j.ntt.2013.03.061⟩ |
| Popis: | Ortho-substituted polychlorinated biphenyls (PCBs) preferentially accumulate in the brain and cerebrospinal fluid (CSF) compared to other PCBs. We previously reported that the same dose of 2,2',4,4',5,5'-hexachlorobiphenyl (PCB153) induced higher concentrations in the CSF of treated animals compared to controls during short days (SD), while no differences were observed during long days (LD). Similarly, the plasma concentration of PCB153 in treated ewes was higher during SD than LD. To understand the structural and molecular events explaining the photoperiodically different concentration of PCBs in the CSF in sheep, we studied the effect of photoperiod on PCB153 action on tight junction (TJ) protein expression in the choroid plexus (CP) of ewes. For that purpose, we collected CP from ovariectomised, estradiol-treated ewes maintained under artificial LD or SD and orally administered with a low dose (0.33 mg/kg/day, 3 times per week for 3 weeks) of PCB153 or vehicle. Exposure to PCB153 affected TJ proteins only during SD, and the levels of claudin-1, zonula occludens-2 (ZO-2), and afadin (AF-6) were significantly lower when compared to vehicle-treated animals. No differences were observed for occludin, junctional adhesion molecule-1 (JAM-1), claudin-5, ZO-1 and ZO-3. There was no effect of PCB153 treatment on TJ-mRNA levels. These results indicate that PCB153 selectively alters TJ proteins in the ovine CP. These alterations appear to be associated with the level of PCB153 in the blood plasma, which is modulated by the photoperiod. This study emphasises the importance of photoperiod in the susceptibility of adult sheep to PCBs. |
| Databáze: | OpenAIRE |
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