The application of graphene oxidized combining with decellularized scaffold to repair of sciatic nerve injury in rats
| Autor: | Qiaoling Wang, Xiao-Jie Tong, Jinlong Chen, Xiaohong Sun, Qingfei Niu, Xiumei Fu |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Action potential Scanning electron microscope Pharmaceutical Science Infrared spectroscopy 02 engineering and technology 010402 general chemistry 01 natural sciences Article law.invention law medicine Axon Pharmacology Graphene lcsh:RM1-950 Anatomy Sciatic nerve injury 021001 nanoscience & nanotechnology medicine.disease 0104 chemical sciences lcsh:Therapeutics. Pharmacology medicine.anatomical_structure Transmission electron microscopy Sciatic nerve 0210 nano-technology Biomedical engineering |
| Zdroj: | Saudi Pharmaceutical Journal, Vol 25, Iss 4, Pp 469-476 (2017) |
| ISSN: | 1319-0164 |
| DOI: | 10.1016/j.jsps.2017.04.008 |
| Popis: | This paper combined the decellularized scaffold of sciatic nerve of rats with graphene oxidized (GO), studied and facilitated the regeneration of sciatic nerve of rats, and provided the basis for the clinical application of nanomaterials. GO was prepared through improving Hammer’s Method. Fourier Infrared Spectrum was used to scan and detect the functional groups in GO of sample by using the pellet method, the microcosmic morphological appearance of GO was observed by using the scanning electron microscope. The GO/decellularized scaffold were prepared and operation bridging of injured sciatic nerve was conducted by using the oscillation mixing method. BL-420F Biofunctional Experiment System was used to detect nerve action potential and the maximum tension value of muscles, and the fiber structure of nerve was observed under H-7650 Transmission Electron Microscope (TEM). Scanning electron microscope observed that GO presented a folded and curly single-layer sheet structure. It was soluble in water through ultrasound, brownish, the Fourier Transform Infrared Spectrometer detected the absorption peaks of carbonyl, hydroxy and carboxy, proving that the surface of GO material had many functional groups containing oxygen. Decellularized scaffold combining with GO was applied to repair injury of sciatic nerve, the nerve action potential, maximum tension value of muscle, wet weight value of gastrocnemius, thickness of gastrocnemius, thickness of myelin sheath and diameter of axon of the decellularized scaffold combining with GO group were obviously higher than the decellularized scaffold group and the self-rotating group, approaching to the normal value. All the data were represented by means ± standard deviation (x¯±s) and processed by adopting SPSS 11.0 software. Comparisons among groups were analyzed by variance, and the comparison of two means was detected by student t. The detection level adopted α = 0.05, when P |
| Databáze: | OpenAIRE |
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