Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
| Autor: | Calvin C L Cheung, Wafa' T Al-Jamal, Amalia Ruiz, Guanglong Ma |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Indocyanine Green
Ammonium sulfate Doxorubicin loading (DOX) Chemistry Pharmaceutical General Chemical Engineering Microfluidics Size-exclusion chromatography Indocyanine green (ICG) Micromixer Bioengineering 02 engineering and technology Buffers LTSLs 01 natural sciences Staggered herringbone micromixer General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Drug Delivery Systems Issue 157 Lab-On-A-Chip Devices 0103 physical sciences medicine Doxorubicin Chromatography Thermosensitive Liposome Ethanol 010304 chemical physics General Immunology and Microbiology General Neuroscience Temperature Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Lipids Lysolipid Solvent chemistry Ammonium Sulfate Cholesterol-free Liposomes 0210 nano-technology medicine.drug |
| Zdroj: | Cheung, C C L, Ma, G, Ruiz, A & Al-Jamal, W T 2020, ' Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes ', Journal of visualized experiments : JoVE, vol. 157, no. 157, e60907 . https://doi.org/10.3791/60907 |
| ISSN: | 1940-087X |
| DOI: | 10.3791/60907 |
| Popis: | The presented protocol enables a high-throughput continuous preparation of low temperature-sensitive liposomes (LTSLs), which are capable of loading chemotherapeutic drugs, such as doxorubicin (DOX). To achieve this, an ethanolic lipid mixture and ammonium sulfate solution are injected into a staggered herringbone micromixer (SHM) microfluidic device. The solutions are rapidly mixed by the SHM, providing a homogeneous solvent environment for liposomes self-assembly. Collected liposomes are first annealed, then dialyzed to remove residual ethanol. An ammonium sulfate pH-gradient is established through buffer exchange of the external solution by using size exclusion chromatography. DOX is then remotely loaded into the liposomes with high encapsulation efficiency (> 80%). The liposomes obtained are homogenous in size with Z-average diameter of 100 nm. They are capable of temperature-triggered burst release of encapsulated DOX in the presence of mild hyperthermia (42 °C). Indocyanine green (ICG) can also be co-loaded into the liposomes for near-infrared laser-triggered DOX release. The microfluidic approach ensures high-throughput, reproducible and scalable preparation of LTSLs. |
| Databáze: | OpenAIRE |
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