Characterization of Local and Systemic Impact of Whitefly (Bemisia tabaci) Feeding and Whitefly-Transmitted Tomato Mottle Virus Infection on Tomato Leaves by Comprehensive Proteomics
| Autor: | Wardatou Boukari, Natalia Lucinda, Aaron J. Ogden, Alba Nava, Joshua N. Adkins, Garry Sunter, Jane E. Polston, Wayne R. Curtis |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plasmodesma Whitefly tomato Proteomics 01 natural sciences Catalysis Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences proteomics RNA directed DNA methylation whitefly Physical and Theoretical Chemistry Molecular Biology Gene RNA-Directed DNA Methylation lcsh:QH301-705.5 Spectroscopy biology pathogenesis Organic Chemistry Begomovirus fungi food and beverages General Medicine Bemisia tabaci biology.organism_classification Computer Science Applications Horticulture 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Geminivirus Tomato mottle virus transcriptional gene silencing Solanum tomato mottle virus 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences, Vol 21, Iss 7241, p 7241 (2020) International Journal of Molecular Sciences Volume 21 Issue 19 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Tomato mottle virus (ToMoV) is a single-stranded DNA (ssDNA) begomovirus transmitted to solanaceous crops by the whitefly species complex (Bemisia tabaci), causing stunted growth, leaf mottling, and reduced yield. Using a genetic repertoire of seven genes, ToMoV pathogenesis includes the manipulation of multiple plant biological processes to circumvent antiviral defenses. To further understand the effects of whitefly feeding and whitefly-transmitted ToMoV infection on tomato plants (Solanum lycopersicum &lsquo Florida Lanai&rsquo ), we generated comprehensive protein profiles of leaves subjected to feeding by either viruliferous whiteflies harboring ToMoV, or non-viruliferous whiteflies, or a no-feeding control. The effects of whitefly feeding and ToMoV infection were measured both locally and systemically by sampling either a mature leaf directly from the site of clip-cage confined whitefly feeding, or from a newly formed leaf 10 days post feeding (dpf). At 3 dpf, tomato&rsquo s response to ToMoV included proteins associated with translation initiation and elongation as well as plasmodesmata dynamics. In contrast, systemic impacts of ToMoV on younger leaves 10 dpf were more pronounced and included a virus-specific change in plant proteins associated with mRNA maturation and export, RNA-dependent DNA methylation, and other antiviral plant processes. Our analysis supports previous findings and provides novel insight into tomato&rsquo s local and systemic response to whitefly feeding and ToMoV infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|