The third exoplasmic loop of the thyrotropin receptor is partially involved in signal transduction
| Autor: | Toru Mori, Shinji Kosugi |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
endocrine system
medicine.medical_specialty endocrine system diseases Adrenergic receptor Inositol Phosphates Mutant DNA Mutational Analysis Molecular Sequence Data Biophysics Thyrotropin Transfection Biochemistry Thyrotropin receptor Structure-Activity Relationship Structural Biology Internal medicine Genetics medicine Cyclic AMP Animals G protein-coupled receptor Amino Acid Sequence Receptor PIP2 signal Molecular Biology Peptide sequence cAMP signal Chemistry Receptors Thyrotropin Cell Biology Recombinant Proteins Dual signaling Cell biology Rats Loop (topology) Endocrinology Mutagenesis Receptors Adrenergic beta-2 Signal transduction hormones hormone substitutes and hormone antagonists Signal Transduction |
| Zdroj: | FEBS letters. 349(1) |
| ISSN: | 0014-5793 |
| Popis: | We characterized the role of the three exoplasmic loops in the transmembrane region of the thyrotropin receptor (TSHR) by substituting each loop with the counterpart of β 2 -adrenergic receptor amino acid sequence. Mutant El3 with a substitution of the 3rd exoplasmic loop showed significant decrease in the maximal level and sensitivity of TSH-stimulated cAMP response despite good retention of TSH binding. These findings suggest that the third exoplasmic loop of the TSH receptor is partially involved in the signal transduction mechanism. |
| Databáze: | OpenAIRE |
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