Anti-angiogenic effect of hexahydrocurcumin in rat corneal neovascularization
| Autor: | San-Ni Chen, Chien-Hsing Lee, Chien-Neng Kuo, Jou-Cheng Huang, Li-Ju Lai, Chung-Yi Chen, Chien-Hsiung Lai, Chia-Hui Hung, Ching-Hsein Chen |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A 0301 basic medicine Pathology medicine.medical_specialty Curcumin Angiogenesis medicine.medical_treatment Blotting Western Angiogenesis Inhibitors Fibroblast growth factor Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Western blot Stroma Animals Medicine Corneal Neovascularization medicine.diagnostic_test business.industry Growth factor HLA-DR Antigens medicine.disease Rats Disease Models Animal Ophthalmology Vascular endothelial growth factor A 030104 developmental biology Corneal neovascularization 030221 ophthalmology & optometry Immunohistochemistry Fibroblast Growth Factor 2 business Conjunctiva |
| Zdroj: | International Ophthalmology. 38:747-756 |
| ISSN: | 1573-2630 0165-5701 |
| DOI: | 10.1007/s10792-017-0530-6 |
| Popis: | This study was to investigate the anti-angiogenic effect of hexahydrocurcumin (HHC) to evaluate gene (p-basic fibroblast growth factor (bFGF)-SAINT-18 & p-vascular endothelial growth factor (VEGF)-SAINT-18 complex)-induced corneal neovascularization (CorNV) in rats. CorNV was induced in 24 eyes of 24 rats. Four groups (Group A: 0 μg, B: 0.01 μg, C: 0.1 μg, and D: 1 μg) of HHC were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus at temporal side. The 1 μg of p-bFGF-SAINT-18 & p-VEGF-SAINT-18 complex were prepared and implanted into the rat corneal stroma 1.5 mm from the limbus at the same side. Inhibition of CorNV was observed and quantified from day 1 to day 60. bFGF and VEGF protein expression were analyzed by biomicroscopic examination, western blot analysis, and immunohistochemistry. Subconjunctival injection by 1 μg HHC successfully inhibited gene-induced CorNV in rats. bFGF and VEGF protein expression were reduced after 6 days. Meanwhile, the reduction of HLA-DR expression was detected. Our study showed that the HHC might provide an important anti-angiogenesis factor to inhibit CorNV development at the corneal experimental angiogenesis model. |
| Databáze: | OpenAIRE |
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