Hybrid Flow Bioreactor with All Catalysts Immobilized for Enzymatic Electrosynthesis

Autor: Wassim El Housseini, François Lapicque, Steve Pontvianne, Neus Vilà, Ievgen Mazurenko, Alain Walcarius, Mathieu Etienne
Přispěvatelé: Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Réactions et Génie des Procédés (LRGP), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Bioénergétique et Ingénierie des Protéines (BIP ), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), CNRS, European Regional Development Fund (ERDF)
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: ChemElectroChem
ChemElectroChem, 2022, 9 (16), ⟨10.1002/celc.202200463⟩
ISSN: 2196-0216
Popis: International audience; The electrochemical regeneration of the NADH cofactor was realized in a hybrid flow reactor coupling fuel cell technology and redox flow device, paying attention to the robust immobilization of all catalysts. The rhodium catalyst Rh(Cp∗)(bpy)Cl+ was covalently immobilized on a MWCNT layer and the association with the gas diffusion electrode was carefully optimized. High stability and activity of the electrochemical system were assessed by cyclic voltammetry and amperometry in the flow reactor. Afterwards, the optimal cofactor regeneration was applied to NADH-dependent biosynthesis using immobilized lactate dehydrogenase for the conversion of pyruvate to lactate in the flow cell in the presence of cofactor concentration as low as 10 μM. 79 % faradaic efficiency was achieved and remarkable total turnover number (TTN) were reached: 2500, 18000, and 180000, for the NADH cofactor, the Rh complex and the LDH enzyme, respectively.
Databáze: OpenAIRE
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